The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos
El-Sayed, A., Gad, A., AboelEla, M., Ashour, G. (2017). The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos. EKB Journal Management System, 8(5), 97-100. doi: 10.21608/jappmu.2017.45784
A. El-Sayed; A. Gad; M. AboelEla; G. Ashour. "The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos". EKB Journal Management System, 8, 5, 2017, 97-100. doi: 10.21608/jappmu.2017.45784
El-Sayed, A., Gad, A., AboelEla, M., Ashour, G. (2017). 'The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos', EKB Journal Management System, 8(5), pp. 97-100. doi: 10.21608/jappmu.2017.45784
El-Sayed, A., Gad, A., AboelEla, M., Ashour, G. The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos. EKB Journal Management System, 2017; 8(5): 97-100. doi: 10.21608/jappmu.2017.45784

The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos

Journal of Animal and Poultry Production
Article 2, Volume 8, Issue 5, May 2017, Page 97-100  XML PDF (283.66 K)
Document Type: Original Article
DOI: 10.21608/jappmu.2017.45784
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Authors
A. El-Sayed1; A. Gad1; M. AboelEla1; G. Ashour2
11 Department of Animal Production, Faculty of Agriculture,Cairo University,12613, Giza, Egypt. 2 Embryology and Cell Culture Research Laboratory, Cairo University Research Park (CURP), Faculty of Agriculture, 12613 Giza, Egypt.
2Department of Animal Production, Faculty of Agriculture,Cairo University,12613, Giza, Egypt
Abstract
Embryo cryopreservation becomes a pivotal side in the shape of assisted reproductive technologies. It plays an essential role in several cases such as, embryo storing during transportation, embryo preservation for future usesand establishment ofcryobanks for endangered species and rare breeds. The presentexperiment was conducted to evaluate the cryotoleranceofin vitro produced buffalo embryosat blastocyst stage to vitrification process using cryotop. Embryos were produced using conventional in vitro fertilisation technique and had been divided into two categories: non-vitrified group, which was culturedin vitro till hatched blastocyst stage and vitrified group, which was collected at day 7 for cryopreservation. A stepwise vitrification and thawing procedures were performed using Cryotop. Survival rate of embryos was observed within 4 hours of thawing, then the ability to expand and hatch was recorded.The expansion rate was higher in control group compared to vitrified group (91.66 vs. 86.36%, respectively) with no significant differences. In both groups, all expanded blastocysts reached the hatching stage normally.Therefore, our results suggested thatCryotop is aneffective tool for cryopreservation of in vitro produced Egyptian buffalo blastocyst.
Keywords
Buffalo; embryo; Vitrification; Cryotop; In vitro fertilisation
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