Isolation and Molecular Diagnosis of Bacteria from the Contact Lenses of Women | ||
| Journal of Bioscience and Applied Research | ||
| Article 9, Volume 11, Issue 4, November 2025, Pages 1157-1171 PDF (626.57 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jbaar.2025.374691.1183 | ||
| Authors | ||
| AMJAD IBRAHIM Oraibi* 1; Mohammed Al-SAEDI2; Mohammed Jabbar3; rusul Jabbar4; Ali Adnan5 | ||
| 1Almanara college for medical sciences | ||
| 2Misan Health Directorate, Department of forensic medicine | ||
| 3Misan Health Directorate, Department of Forensic Medicine | ||
| 4Al-Manara College for Medical Sciences, Misan, Iraq ,62001 | ||
| 5Al-Manara College for Medical Sciences, Misan, Iraq ,62001 and Misan Health Directorate, AL-sader Teaching Hospital , Misan, Iraq | ||
| Abstract | ||
| This study aimed to isolate and identify bacterial pathogens responsible for eye inflammation in contact lens customers. From the lens fluids, 25 samples were collected. Laboratory analysis revealed bacterial growth in 15 samples. The predominant bacteria identified were Staphylococcus aureus (Gram-positive) and Pseudomonas spp. (Gram-negative), each accounting for 33% of positive samples. Other isolates included Streptococcus pneumoniae and Staphylococcus epidermidis (Gram-positive), found in 13% of cases, while Escherichia coli (Gram-negative) was detected in 6.6%. The study further evaluated antibiotic sensitivity, demonstrating that Cefazolin and Cefalexin exhibited high efficacy against all isolated bacteria. In contrast, Penicillin and Amoxicillin showed resistance across all bacterial strains tested. These findings highlight the importance of proper hygiene among contact lens users and suggest Cefazolin and Cefalexin as effective treatment options for bacterial eye infections associated with contact lens use. | ||
| Keywords | ||
| contact lenses; E. coli; Gram stain; antibiotics; and samples | ||
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