STUDY OF RELATIONSHIP BETWEEN SP1 POLYMORPHISM IN THE COLLAGEN TYPE I ALPHA -1 (COLIA1) GENE AND OSTEOPROSIS IN PATIENTS WITH BETA-THALASSEMIA.
Khalifa, N., Hesham, M., Elbaz, N., Omran, A., AbdElmonem., D. (2017). STUDY OF RELATIONSHIP BETWEEN SP1 POLYMORPHISM IN THE COLLAGEN TYPE I ALPHA -1 (COLIA1) GENE AND OSTEOPROSIS IN PATIENTS WITH BETA-THALASSEMIA.. EKB Journal Management System, 23(1), 1-13. doi: 10.21608/zumj.2017.4680
Naglaa Khalifa; Mervat Hesham; Nadia Elbaz; Alaa Omran; Doaa AbdElmonem.. "STUDY OF RELATIONSHIP BETWEEN SP1 POLYMORPHISM IN THE COLLAGEN TYPE I ALPHA -1 (COLIA1) GENE AND OSTEOPROSIS IN PATIENTS WITH BETA-THALASSEMIA.". EKB Journal Management System, 23, 1, 2017, 1-13. doi: 10.21608/zumj.2017.4680
Khalifa, N., Hesham, M., Elbaz, N., Omran, A., AbdElmonem., D. (2017). 'STUDY OF RELATIONSHIP BETWEEN SP1 POLYMORPHISM IN THE COLLAGEN TYPE I ALPHA -1 (COLIA1) GENE AND OSTEOPROSIS IN PATIENTS WITH BETA-THALASSEMIA.', EKB Journal Management System, 23(1), pp. 1-13. doi: 10.21608/zumj.2017.4680
Khalifa, N., Hesham, M., Elbaz, N., Omran, A., AbdElmonem., D. STUDY OF RELATIONSHIP BETWEEN SP1 POLYMORPHISM IN THE COLLAGEN TYPE I ALPHA -1 (COLIA1) GENE AND OSTEOPROSIS IN PATIENTS WITH BETA-THALASSEMIA.. EKB Journal Management System, 2017; 23(1): 1-13. doi: 10.21608/zumj.2017.4680

STUDY OF RELATIONSHIP BETWEEN SP1 POLYMORPHISM IN THE COLLAGEN TYPE I ALPHA -1 (COLIA1) GENE AND OSTEOPROSIS IN PATIENTS WITH BETA-THALASSEMIA.

Zagazig University Medical Journal
Article 4, Volume 23, Issue 1, January 2017, Page 1-13  XML PDF (944.19 K)
Document Type: Original Article
DOI: 10.21608/zumj.2017.4680
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Authors
Naglaa Khalifa1; Mervat Hesham1; Nadia Elbaz2; Alaa Omran2; Doaa AbdElmonem.
1Clinical Pathology and pediatrics Medicine Departments, Faculty of Medicine, Zagazig University; Egypt.
2Clinical Pathology and pediatrics Medicine Departments, Faculty of Medicine, Zagazig University; Egypt
Abstract
Background:-Osteoporosis is an important cause of morbidity in patients with β-thalassemia. The pathogenesis of reduced bone mineral density (BMD) is multifactorial. A range of genetics factors have been implicated in patients with osteoporosis. Polymorphism at the Sp1 binding site of the collagen type I A 1(COLIA1) gene is thought to be an important factor in development of osteoporosis. Objective:-is to study the relationship between SP1 polymorphism in the collagen type 1 alpha 1 gene and the development of osteoporosis in patients with Beta thalassemia. Methods:-A total of 40 patients with β-thalassemia(21 females &19 males)aged 6-18 yearsand 40 healthy subjects cross matched with age and sex. Serum osteocalcin, serum alkaline phosphatase, calcium and DEXA were examined in all patients. The COLIA1 Sp1genotypes (SS, Ss, ss) were measured by digestion using restriction enzyme (Bal1) of DNA amplified by the polymerase chain reaction (PCR). Results:-SS (G/G) genotype in β-thalassemia major was30.7% and in β-thalassemia intermedia was 49.4% and the Ss (G/T) genotype in βT Major was 55.63% and in βT intermedia was 50.6%, the ss genotype (T/T) type in βTM was 13.67% only. The difference was statistically significant (p= 0.040). There was highly significant difference between thalassemia major and thalassemia intermedia compared to control group as regard Ca, OC, ALP and DEXA (P= 0.000). Conclusions:-Detection base substitutions at the Sp1 binding site on the COLIA1 gene in early years considering an important role in preventing osteoporosis in children with beta -thalassemia.and early mangment of these patients.
Keywords
Beta-Thalassemia; COLIA1; Osteoprosis
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