ASSESSMENT OF RAPD MARKERS VARIABILITY OF LOCAL TURKEY (MELEAGRIS GALLOPAVO) POPULATION IN EGYPT | ||||
| Egyptian Poultry Science Journal | ||||
| Article 2, Volume 39, Issue 3, September 2019, Page 615-624 PDF (721.31 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/epsj.2019.46940 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| M. Helal Mostafa | ||||
| Dep. of Anim. Prod., Fac. of Agric., Cairo Uni., 12613 Giza, Egypt | ||||
| Abstract | ||||
| This study was performed to assess the diversity level within the local turkey population in Egypt in comparison with the commercial turkeys. Ten mature males and ten mature females of each population were used. RAPD-PCR technique was applied using ten 10-mer random primers. Altogether 2790 bands were detected, with an average of 6.975 bands overall individuals and primers. In general, the amplified bands were higher in commercial individuals (1695 bands) than the local ones (1690 bands). The total fragment number (TFN) was 115 with an average of 11.5 fragment/primer. The average of PIC percentage of 59% overall individuals. Nevertheless, no specific or unique band was detected. Shannon information index was higher in Baladi (0.615) than in commercial turkeys (0.488). Shannon diversity index also was higher in Baladi (0.433) compared to commercial turkeys (0.328). Analysis of molecular variance (AMOVA) showed that 79% of the total variation was attributed to the within-population variance. The principal component analysis (PCA) results showed similar trend, 83.3% of the total variance was explained by the first 3 axes partitioned to 75.96%, 4.46% and 2.88% for the first, second and third axis, respectively. The study highlighted the high level of within-population genetic variability in the local Egyptian turkeys. | ||||
| Keywords | ||||
| Diversity-local turkeys-Meleagris gallopavo-PIC-RAPD-PCR | ||||
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