Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria
Negm El-Dein, A., Noor El-Deen, A., Tolba, S., El-Shatoury, E., Awad, G., Ibrahim, M., Farid, M. (2017). Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria. EKB Journal Management System, 52(1), 87-100. doi: 10.21608/ejm.2017.1336.1025
Asmaa Negm El-Dein; Azza Noor El-Deen; Sahar Tolba; Einas El-Shatoury; Ghada Awad; Mohamed Ibrahim; Mohamed Farid. "Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria". EKB Journal Management System, 52, 1, 2017, 87-100. doi: 10.21608/ejm.2017.1336.1025
Negm El-Dein, A., Noor El-Deen, A., Tolba, S., El-Shatoury, E., Awad, G., Ibrahim, M., Farid, M. (2017). 'Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria', EKB Journal Management System, 52(1), pp. 87-100. doi: 10.21608/ejm.2017.1336.1025
Negm El-Dein, A., Noor El-Deen, A., Tolba, S., El-Shatoury, E., Awad, G., Ibrahim, M., Farid, M. Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria. EKB Journal Management System, 2017; 52(1): 87-100. doi: 10.21608/ejm.2017.1336.1025

Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria

Egyptian Journal of Microbiology
Article 7, Volume 52, Issue 1, December 2017, Page 87-100  XML PDF (1.02 MB)
Document Type: Original Article
DOI: 10.21608/ejm.2017.1336.1025
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Authors
Asmaa Negm El-Dein email 1; Azza Noor El-Deen1; Sahar Tolba2; Einas El-Shatoury2; Ghada Awad1; Mohamed Ibrahim2; Mohamed Farid1
1Chemistry of Natural & Microbial Products Department, National Research Center, Giza, Egypt
2Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
EIGHT lactic acid bacterial (LAB) isolates were obtained from food and non-food sources and identified by 16S rRNA gene sequence analysis. Based on the sequencing results, the isolates belong to two species of lactobacilli, Lactobacillus plantarum and L. rhamnosus. These strains were then compared with a reference strain, Lactobacillus casei, to assess various probiotic properties, such as haemolytic activity, histamine formation ability, stress tolerance under certain stress conditions, antibiotic susceptibility and in vitro adhesion ability. Moreover, bile salt hydrolase (BSH) activity was evaluated both qualitatively and quantitatively. The results showed that none of the isolates demonstrated any haemolytic activity or histamine formation. The isolated strains were also tolerant to acidic and alkaline conditions (pH 2.5, 3.5 and 9) for 3 and 6 h, as well as osmotic (3 M NaCl) and heat (55 and 70°C) stress, but were more responsive to oxidative and bile stress. The bacterial isolates also expressed high amounts of BSH, ranging from 90 to 142 U/mg in active cells, compared to L. casei (74 U/mg), which may be useful in cholesterol reduction. All bacterial isolates were resistant to vancomycin and susceptible to amoxicillin, cloxacillin and penicillin. All isolates were also highly hydrophobic (>70%), indicating that they are not easily flushed from the intestines.
Keywords
LAB; Probiotic properties; Stress tolerance; Bile salt hydrolase
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