Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells
Mohamed, W., Rabeea, O., Abu Shady, H., Youssef, H. (2017). Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells. EKB Journal Management System, 52(1), 101-111. doi: 10.21608/ejm.2017.1272.1024
Wafaa Farouk Mohamed; Omar Rabeea; Hala Abu Shady; Hebat Allah Youssef. "Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells". EKB Journal Management System, 52, 1, 2017, 101-111. doi: 10.21608/ejm.2017.1272.1024
Mohamed, W., Rabeea, O., Abu Shady, H., Youssef, H. (2017). 'Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells', EKB Journal Management System, 52(1), pp. 101-111. doi: 10.21608/ejm.2017.1272.1024
Mohamed, W., Rabeea, O., Abu Shady, H., Youssef, H. Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells. EKB Journal Management System, 2017; 52(1): 101-111. doi: 10.21608/ejm.2017.1272.1024

Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells

Egyptian Journal of Microbiology
Article 8, Volume 52, Issue 1, December 2017, Page 101-111  XML PDF (859.48 K)
Document Type: Original Article
DOI: 10.21608/ejm.2017.1272.1024
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Authors
Wafaa Farouk Mohamed email 1; Omar Rabeea2; Hala Abu Shady2; Hebat Allah Yousseforcid 2
1Microbiology Department, Ain Shams University Specialized Hospital, Carior, Egypt
2Microbiology Department,Faculty of Science, Ain Shams University, Cairo. Egypt
Abstract
I N THE PRESENT study, we first aimed to select some potential anti-cancer probiotics in vitro.Using the inulin fermentation product by the selected five probiotic bacteria (Lactobacillus paraplantarum strain S143, L. pentosus strain P2-3, L. plantarum strain J12, L.gasseri strain F. and L.reuteri strain IRT), Caco-2 cells were exposed to 50% filtrate supernatant of each probiotic bacteria after 24 h of incubation. Results showed that inulin fermentation product of the selected probiotics cause apoptosis in the Caco-2 cell line. Also, the viability of the freeze-dried powders of the five probiotics using skimmed milk as cryoprotectant was followed up for 12 weeks of storage at refrigerated conditions and also at room temperature. Results proved that lyophilized powders showed slight decrease in their viable count during storage both at room and refrigerated temperatures. This would ensure a good cell concentration for at least 3 months to be administered as a probiotic and would guarantee the long-term delivery of sufficient active, viable, and functional cultures.
Keywords
probiotic; Mixture; Freeze dried; synbiotic; Apoptosis and Tumor
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