Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds
Metwally, E., Madboly, A., Farag, A., Abdelaziz, T., Farag, H. (2018). Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds. EKB Journal Management System, 26(2), 83-99. doi: 10.21608/mjfmct.2018.47195
Eslam S Metwally; Abdelmonem Madboly; Amina Farag; Taghreed A Abdelaziz; Hesham A Farag. "Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds". EKB Journal Management System, 26, 2, 2018, 83-99. doi: 10.21608/mjfmct.2018.47195
Metwally, E., Madboly, A., Farag, A., Abdelaziz, T., Farag, H. (2018). 'Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds', EKB Journal Management System, 26(2), pp. 83-99. doi: 10.21608/mjfmct.2018.47195
Metwally, E., Madboly, A., Farag, A., Abdelaziz, T., Farag, H. Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds. EKB Journal Management System, 2018; 26(2): 83-99. doi: 10.21608/mjfmct.2018.47195

Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds

Mansoura Journal of Forensic Medicine and Clinical Toxicology
Article 7, Volume 26, Issue 2, July 2018, Page 83-99  XML PDF (1.12 MB)
Document Type: Original Article
DOI: 10.21608/mjfmct.2018.47195
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Authors
Eslam S Metwally1; Abdelmonem Madboly email 2; Amina Farag1; Taghreed A Abdelaziz3; Hesham A Farag4
1Forensic Medicine & Clinical Toxicology Department, Faculty of Medicine, Benha University, Egypt.
2Forensic Medicine & Clinical Toxicology Department, Faculty of Medicine, Benha University, Egypt.
3Pathology Department, Faculty of Medicine, Benha University, Egypt.
4Chief Medical Examiner, Forensic Medicine Authority, Ministry of Justice, Egypt.
Abstract
Fibronectin and P-selectin are immunohistochemical markers of skin wounds vitality and age estimation. This study aimed to assess and compare between fibronectin and P-selectin as markers of vitality and dating of antemortem (AM) and postmortem (PM) human skin wounds. This study was carried out on 140 human cases that were classified into three main groups; Group I [90 cases received at the Emergency Department, Benha University Hospital, Egypt], was sub-classified according to the wound age: AM1 (<30minutes; n = 30), AM2 (30-60 minutes; n = 30), and AM3 (60-90 minutes; n = 30); Group II [40 cadavers received at Zenhum Morgue, Ministry of Justice, Egypt], was sub-classified according to the postmortem interval: PM1 (≤12 hours; n =20), PM2 (>12 hours; n =20) and Group III [10 normal control skin samples]. Immunohistochemical (IHC) staining technique was performed for all full thickness skin biopsies. The current study confirmed that Pselectin and fibronectin were good markers of wound vitality and designed a scoring system based on the increased intensity of the IHC staining in relation to wound age.The current study concluded that P-selectin is more reliable and specific than fibronectin as a marker of wound age estimation and vitality.
Keywords
Fibronectin; P-selectin; Immunohistochemical; Wound vitality; Scoring System; Wound age
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