Circulating Angiogenic microRNA-27b and Thrombospondin-1 (TSP-1) Level in Patients with Diabetic Retinopathy | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 8, Volume 77, Issue 2, October 2019, Page 4925-4932 PDF (616.81 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2019.47602 | ||||
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| Authors | ||||
| Samia Souka Moustafa1; Maisa Nour El-Din Shawkey1; Aida Ahmed Abd Elhameed1; Mohamed Maher EL Hefny2; Raghda Abd-Elsalam Nagaty Hanady* 1 | ||||
| 1Department of Chemical Pathology, Faculty of Medicine, Al-Azhar University | ||||
| 2Department of Ophthalmology, Research Institute of Ophthalmology-Ministry of Scientific Research | ||||
| Abstract | ||||
| Background: Diabetes mellitus (DM) is a chronic disease characterized by insulin deficiency or its peripheral resistance resulting in hyperglycemia and non-enzymatic glycation of protein.The chronic hyperglycemia of diabetes is associated with long-term damage, dysfunction, and failure of different organs, especially the eyes, kidneys, nerves, heart, and blood vessels. MicroRNAs (miRNAs) are a class of highly conserved 19–25 nucleotide noncoding RNAs that regulate gene expression. Objective:This study aimed to assess plasma miR-27b expression in diabetic retinopathy patients as an early diagnostic parameter in both proliferative and non-proliferative and to assess the correlation between plasma levels of miR-27b with the clinico-pathological parameters of diabetic patients as well as determination of the level of thrombospondin-1 (TSP1). Patients and Methods: This study was conducted on 60 diabetic retinopathy Egyptian patients that were recruited from the Research Institute of Ophthalmology - RIO (Medical Retina Clinics) during their routine screening for diabetic retinopathy. Results: Micro RNA 27b shows a highly significant difference (increase) among PDR compared to non-PDR & control groups (P value: 0.001) and shows a moderately significant increase in expression in PDR group compared to control group (P value: 0.01). This significant increase in microRNA between PDR group and control group has the ability of being a non-invasive biomarker for diagnosis of PDR versus control group, as miRNA-27b expression showed AUC = 0.889 with (95% CI 0.744–1.000). The best cut-off point of miRNA-27b is 1.95 with 88.9% sensitivity & 100% specificity and the p value <0.001(highly significant). Conclusion: TSP-1 can act as a predictor for transformation of non-retinopathy stage to PDR stage. miRNA-27b has the ability to differentiate between the PDR and control group and could be considered a non invasive biomarker. | ||||
| Keywords | ||||
| Diabetes mellitus; microRNA-27b; Thrombospondin-1; diabetic retinopathy | ||||
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