Role of Some Enzymes Produced by Egyptian Bacterial Isolate on Decolorization of Blue and Yellow Textile Dyes
Ahmed, A., Abou-Taleb, K., Ebeed, N., Khalil, H. (2019). Role of Some Enzymes Produced by Egyptian Bacterial Isolate on Decolorization of Blue and Yellow Textile Dyes. EKB Journal Management System, 54(1), 77-89. doi: 10.21608/ejm.2019.15326.1107
Alshaymaa I Ahmed; Khadiga Abou-Taleb; Naglaa Ebeed; Hala Bader Khalil. "Role of Some Enzymes Produced by Egyptian Bacterial Isolate on Decolorization of Blue and Yellow Textile Dyes". EKB Journal Management System, 54, 1, 2019, 77-89. doi: 10.21608/ejm.2019.15326.1107
Ahmed, A., Abou-Taleb, K., Ebeed, N., Khalil, H. (2019). 'Role of Some Enzymes Produced by Egyptian Bacterial Isolate on Decolorization of Blue and Yellow Textile Dyes', EKB Journal Management System, 54(1), pp. 77-89. doi: 10.21608/ejm.2019.15326.1107
Ahmed, A., Abou-Taleb, K., Ebeed, N., Khalil, H. Role of Some Enzymes Produced by Egyptian Bacterial Isolate on Decolorization of Blue and Yellow Textile Dyes. EKB Journal Management System, 2019; 54(1): 77-89. doi: 10.21608/ejm.2019.15326.1107

Role of Some Enzymes Produced by Egyptian Bacterial Isolate on Decolorization of Blue and Yellow Textile Dyes

Egyptian Journal of Microbiology
Article 7, Volume 54, Issue 1, 2019, Page 77-89  XML PDF (2.74 MB)
Document Type: Original Article
DOI: 10.21608/ejm.2019.15326.1107
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Authors
Alshaymaa I Ahmed email orcid 1; Khadiga Abou-Taleb2; Naglaa Ebeed3; Hala Bader Khalil3
1Department of Agricultural Microbiology, Faculty of Agriculture, Beni-Suef University Beni-Suef, Egypt
2Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
3Department of Genetics, Faculty of Agriculture, Ain Shams Uniersity, Cairo, Egypt
Abstract
DECOLORIZATION of textile reactive azo dyes, blue and yellow, by 15 bacterial isolates collected from Egyptian arid soil was investigated. Of these, S8 isolate revealed a highest decolorization performance. Here, we identified S8 isolate on a molecular level based on type I chaperonin universal target sequence (cpn60). The most similar DNA sequence to the S8 DNA sequence was for Klebsiella oxytoca partial CDS (AB008147.1) coding for GroES and GroEL chaperon homologues (acronyms of cpn60) with 88% DNA sequence similarity. The impact of numerous exterior parameters to improve the decolorization abilities of this isolate was studied. A maximum decolorization activity occurred at a medium containing glucose, soybean husk at a C/N ratio of 12:1 supplemented with dye concentration of 100mg/L and amended with 3% (v/v) inoculum. Incubation for 4 days at 35°C±2 with shaking at 150rpm reached the decolorization activity to 89.35 and 78.23% for blue and yellow dyes, respectively. The ascending levels of bacterial enzymes like azo-reductase, phenol red manganese peroxidase and ascorbate oxidase indicated their prominent roles in dye degradation.
Keywords
dyes; Decolorization; Degrading enzymes; <i> Klebsiella oxytoca </i>; cpn60; Gene sequence
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