EFFICIENCY OF SOME BIONEMATICIDES AGAINST ROOT-KNOT NEMATODE Meloidogyne incognita ON THREE TOMATO CULTIVARS UNDER GREENHOUSE CONDITIONS | ||||
Zagazig Journal of Agricultural Research | ||||
Article 12, Volume 45, Issue 6, November 2018, Page 2001-2010 PDF (401.99 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/zjar.2018.47739 | ||||
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Authors | ||||
Ahmed M. El-Deeb ![]() | ||||
1Plant Prot. Dept., Fac. Agric., Zagazig Univ., Egypt | ||||
2Genet. Dept., Fac., Agric., Zagazig Univ., Egypt | ||||
3Hortic. Dept., Fac. Agric., Zagazig Univ., Egypt | ||||
Abstract | ||||
This study was carried out to determine the nematicidal efficiency of seven bionematicides (Nema end, Nema cont, Nema clean, Nema K, Bio-zeid, Bio-arc and Nemex) compared with one nematicide (Nemacur 10%) on three cultivars of tomato against the root-knot nematode (RKN) Meloidogyne incognita under greenhouse conditions. The screened tomato cultivars (endless summer, supermarmand and jueblle) were significantly different in their response to nematode infection. Detectable tolerance to nematode infection was recorded with cultivar Endless summer and Juebelle while the least tolerance was recorded with supermarmand cultivar. Number of galls and egg masses were significantly decreased in the investigated cultivars treated with Nemacur, and Nema k, Nema cont and Bio-zeid (82.49, 73.97; 68.48, 50.00; 30.74, 33.34; 61.09, 40.62%), respectively in endless summer, (78.90, 69.92; 73.70, 55.75; 68.51, 19.48; 72.31, 43.38%), respectively, in supermarmand and (71.15, 67.19; 61.90, 40.64; 59.38, 40.64; 41.18, 15.63%) respectively, in Juebelle. Fresh and dry weights of shoot had significantly increased with same components, (5.10, 8.77; 3.34, 7.21; 2.44, 3.24; 2.69, 4.80%) respectively, in Endless summer and (8.03, 5.09; 6.80, 3.96; 4.54, 3.75; 6.02, 2.44%) respectively, in Juebelle compared with untreated plants, while supermarmand was the most susceptible for infection with RKN. Random amplified polymorphic DNA (RAPD) marker using four primers detected polymorphism in DNA in percentage ranged between 33.33 – 75% with total polymorphism 58.33%. Primer OPC-09 gave the highest polymorphism (75%) while OPB-18 gave the lowest polymorphism (33.33%). | ||||
Keywords | ||||
Tomato cultivars; Bionematicides; Meloidygyne incognita; greenhouse; PCR | ||||
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