Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail
El-Dougdoug, N., Hassan, M., Elashkar, E., Ahmed, A., Hazaa, M. (2020). Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail. EKB Journal Management System, 60(1), 185-197. doi: 10.21608/ejbo.2019.7596.1284
Noha El-Dougdoug; Mervet Hassan; Eslam Elashkar; A. I. Ahmed; Mahmoud Hazaa. "Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail". EKB Journal Management System, 60, 1, 2020, 185-197. doi: 10.21608/ejbo.2019.7596.1284
El-Dougdoug, N., Hassan, M., Elashkar, E., Ahmed, A., Hazaa, M. (2020). 'Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail', EKB Journal Management System, 60(1), pp. 185-197. doi: 10.21608/ejbo.2019.7596.1284
El-Dougdoug, N., Hassan, M., Elashkar, E., Ahmed, A., Hazaa, M. Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail. EKB Journal Management System, 2020; 60(1): 185-197. doi: 10.21608/ejbo.2019.7596.1284

Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail

Egyptian Journal of Botany
Article 14, Volume 60, Issue 1, April 2020, Page 185-197  XML PDF (2.71 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2019.7596.1284
View on SCiNiTO View on SCiNiTO
Authors
Noha El-Dougdoug email 1; Mervet Hassan1; Eslam Elashkar2; A. I. Ahmed2; Mahmoud Hazaa email 1
1Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt
2Benha Fever Hospital, Benha, Egypt
Abstract
THE RISE of clinical concern about antibiotic-resistant Salmonella enterica serovar Typhi is mainly attributed to the frequent contamination of food and drinking water. The aim of this study is to assess the sensitivity of S. Typhi to antibiotics as well as isolation of lytic bacteriophages specific to the bacterium and investigate their ability to enhance the safety of tap water and milk. S. Typhi clinical isolates recovered from infected blood specimens were included in this study. The bacterial isolates were identified by conventional biochemical tests; Vitek analyzer in addition to Widal test to determine the serotypes of Salmonella isolates. Four lytic bacteriophages specific to S. Typhi were isolated and characterized using TEM and designated as vb_StyM,EN-1, vb_StyM,EN-2, vb_StyS,EN-3 and vb_StyP,EN-4. The results showed resistance of S. Typhi isolates to various antibiotics. Phage cocktail was prepared and applied for controlling S. Typhi growth in milk and drinking water at 4⁰C and room temperature. After treatment, complete eradication of bacterial growth was observed in broth medium at 4°C and room temperature after 24hrs. In addition to that, phage cocktail showed complete inhibition of S. Typhi growth in treated tap water at 25°C after 24hrs and in milk at 4°C. A significant reduction; nearly five log reductions was observed in skim milk while one log reduction was obtained in full fat milk at 25°C. These results indicate that S. Typhi is resistant to some antibiotics and bacteriophages could be promising antimicrobial alternatives.
Keywords
Antibiotic resistance; Bio-control; Phage cocktail; Salmonella Typhi; Skim milk
Statistics
Article View: 610
PDF Download: 617