EFFICACY OF MOLECULAR DIAGNOSIS TO DETERMINE GENETIC DIVERSITY AMONG INDIGENOUS ISOLATES OF Erwinia carotovora UNDER EGYPTIAN CONDITION
Hassan, E., Refaat, M. (2018). EFFICACY OF MOLECULAR DIAGNOSIS TO DETERMINE GENETIC DIVERSITY AMONG INDIGENOUS ISOLATES OF Erwinia carotovora UNDER EGYPTIAN CONDITION. EKB Journal Management System, 45(6), 2455-2464. doi: 10.21608/zjar.2018.47891
Eman O. Hassan; Mohamed H. Refaat. "EFFICACY OF MOLECULAR DIAGNOSIS TO DETERMINE GENETIC DIVERSITY AMONG INDIGENOUS ISOLATES OF Erwinia carotovora UNDER EGYPTIAN CONDITION". EKB Journal Management System, 45, 6, 2018, 2455-2464. doi: 10.21608/zjar.2018.47891
Hassan, E., Refaat, M. (2018). 'EFFICACY OF MOLECULAR DIAGNOSIS TO DETERMINE GENETIC DIVERSITY AMONG INDIGENOUS ISOLATES OF Erwinia carotovora UNDER EGYPTIAN CONDITION', EKB Journal Management System, 45(6), pp. 2455-2464. doi: 10.21608/zjar.2018.47891
Hassan, E., Refaat, M. EFFICACY OF MOLECULAR DIAGNOSIS TO DETERMINE GENETIC DIVERSITY AMONG INDIGENOUS ISOLATES OF Erwinia carotovora UNDER EGYPTIAN CONDITION. EKB Journal Management System, 2018; 45(6): 2455-2464. doi: 10.21608/zjar.2018.47891

EFFICACY OF MOLECULAR DIAGNOSIS TO DETERMINE GENETIC DIVERSITY AMONG INDIGENOUS ISOLATES OF Erwinia carotovora UNDER EGYPTIAN CONDITION

Zagazig Journal of Agricultural Research
Article 44, Volume 45, Issue 6, November 2018, Page 2455-2464  XML PDF (406.41 K)
Document Type: Original Article
DOI: 10.21608/zjar.2018.47891
View on SCiNiTO View on SCiNiTO
Authors
Eman O. Hassan1; Mohamed H. Refaat email 2
1Pl. Pathol. Dept., Fac. Agric., Benha Univ., Egypt
21-Genet. and Genet. Eng. Dept., Fac. Agric., Benha Univ., Egypt 2-Moshtohor Res. Park, Molecular Biol. Lab., Benha Univ., Egypt
Abstract
Thirty-Four isolates of Erwinia carotovora subspecies carotovora (Ecc) causing soft rot of potato, were collected from tubers of potato plants grown in North and East Egypt. The frequency of the isolated species of soft rot-associated bacteria, being 10, 10, 8 and 6 isolates from Governorates of Sinai, Sharqia, Gharbia and Beheira, respectively. The isolates were identified as Ecc by biochemical method and molecular identity then was confirmed by polymerase chain reaction (PCR) using specific primers FEc1 and REc1 which amplified the Eca-specific band of 690-733 bp. Pathogenicity test divided these 9 isolates into 3 aggressiveness groups, High infection (2 isolates), moderate infection (5 isolates) and low infection (2 isolates), receptively. These isolates were also characterized using the technique of Inter Simple Sequence Repeats polymerase chain reaction (ISSR-PCR) found that 61 amplified fragments, 56 were polymorphic (91.8%) and the percentage of polymorphism ranged from 100% (844B) to 84.62% (HB12); the fragment sizes were 224bp to 1.36 kb. The unweighted pair-group method with arithmetical average (UPGMA) cluster analysis based on pairwise genetic similarity coefficient revealed that the similarity between the selected 9 soft rot bacterial isolates ranged from 0.125 between Ec6 and Ec9 to 0.875 between Ec1, Ec3 and Ec4. The average of similarity among genotypes was 0.500 and divided the soft rot isolates into three well-defined clusters showing a great level of genetic diversity. However, these clusters were not specific to aggressiveness groups, origin or special potato variety. Isolates with different aggressiveness levels, originated from different potato varieties participate between the same clusters. This means that the isolates likely derived from the same source population and got scattered from one area to another through their hosts.
Keywords
Potato (Solanum tuberosum) Erwinia carotovora; soft rot; Genetic diversity; ISSR-PCR
Statistics
Article View: 157
PDF Download: 238