Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats
Aminu, I., Teslimat, A., Victoria, W., Samson, C., Aboyeji, O., Olatunbosun, O., Sheu-Tijani, S., Saliu, A. (2019). Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats. EKB Journal Management System, 57(1), 25-34. doi: 10.21608/BFPC.2019.47261
Imam Aminu; Alakoso Jumoke Teslimat; Williams Victoria; Chengetanai Samson; Oyewole Lukuman Aboyeji; Olaleye Olatunbosun; Shittu Toyin Sheu-Tijani; Ajao Moyosore Saliu. "Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats". EKB Journal Management System, 57, 1, 2019, 25-34. doi: 10.21608/BFPC.2019.47261
Aminu, I., Teslimat, A., Victoria, W., Samson, C., Aboyeji, O., Olatunbosun, O., Sheu-Tijani, S., Saliu, A. (2019). 'Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats', EKB Journal Management System, 57(1), pp. 25-34. doi: 10.21608/BFPC.2019.47261
Aminu, I., Teslimat, A., Victoria, W., Samson, C., Aboyeji, O., Olatunbosun, O., Sheu-Tijani, S., Saliu, A. Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats. EKB Journal Management System, 2019; 57(1): 25-34. doi: 10.21608/BFPC.2019.47261

Nigella sativa oil protected the hippocampus against Acetyl cholinesterase and oxidative dysfunctions-driven impaired working memory in rats

Bulletin of Faculty of Pharmacy, Cairo University
Article 2, Volume 57, Issue 1, 2019, Page 25-34  XML PDF (1.83 MB)
Document Type: Original Article
DOI: 10.21608/BFPC.2019.47261
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Authors
Imam Aminu1; Alakoso Jumoke Teslimat1; Williams Victoria2; Chengetanai Samson2; Oyewole Lukuman Aboyeji3; Olaleye Olatunbosun4; Shittu Toyin Sheu-Tijani5; Ajao Moyosore Saliu1
1Neuroscience Unit, Department of Anatomy, College of Health Sciences, University of Ilorin, Nigeria.
2Comparative Neurobiology Unit, School of Anatomical sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Republic of South Africa.
3Neurophysiology Unit, Department of Physiology, College of Health Sciences, University of Ilorin, Nigeria.
4Department of Oral science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Republic of South Africa.
5Endocrinology and metabolism Research Unit, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Abstract
Oxidative damages in organophosphates poisoning is associated with neuro-cognitive deficits. This study investigates the protective effect of Nigella sativa oil (NSO) in mitigating dichlorvos (DDVP) induced oxidative damage and neuro-cognitive impairment in rats. Thirty-two rats were randomly divided into four groups, exposed to 1 ml/kg of normal saline, 8.8 mg/kg of DDVP, DDVP + 1 ml/kg of NSO and NSO respectively for 14 consecutive days. Body weights were recorded at day 1 and 15 of the experiment, the rats were exposed to 3 trials each on the 11, 12 and 13th days in the Morris water maze, and subsequently latency to hidden platform and time in the platform quadrant were recorded as measures of long term memory (LTM), short term memory (STM) and reference memory on the 14th day. The rats were euthanized on the 15th day, the brains excised and the hippocampi of five brains in each group were removed, homogenized to analyze for total reactive oxygen species (ROS), nitrous oxide (NO) levels and acetylcholinesterase (AChE) activities, while the other three were processed for histology and Ki67 immunohistochemistry. DDVP exposure caused a significant increase in hippocampal NO and ROS levels, with reductions in AChE activities and Ki67 protein expression. This was associated with delayed escape latency and reduced time in platform quadrant. NSO intervention prevented outburst in ROS and NO, preserved the neurogenic cells and improved neuro-cognitive indices. We thus conclude that stabilizing oxidative and neurogenic functions are vital to protect against DDVP hippocampal insults.
Keywords
Acetylcholinesterase; Cognition; dichlorvos; Nigella sativa oil; oxidative damage
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