ENZYME ACTIVITY AND TIME-COURSE QUANTITATIVE DISTRIBUTION OF AN ORAL DOSE OF IMIDACLOPRID IN MALE RAT TISSUES
Nasr, H., Shalaby, A., Ragheb, D., Hendawi, M., Sauvé, S. (2019). ENZYME ACTIVITY AND TIME-COURSE QUANTITATIVE DISTRIBUTION OF AN ORAL DOSE OF IMIDACLOPRID IN MALE RAT TISSUES. EKB Journal Management System, 46(5), 1387-1394. doi: 10.21608/zjar.2019.48158
Heba E. Nasr; A. A. Shalaby; D. A. Ragheb; M. Y. Hendawi; Sébastien Sauvé. "ENZYME ACTIVITY AND TIME-COURSE QUANTITATIVE DISTRIBUTION OF AN ORAL DOSE OF IMIDACLOPRID IN MALE RAT TISSUES". EKB Journal Management System, 46, 5, 2019, 1387-1394. doi: 10.21608/zjar.2019.48158
Nasr, H., Shalaby, A., Ragheb, D., Hendawi, M., Sauvé, S. (2019). 'ENZYME ACTIVITY AND TIME-COURSE QUANTITATIVE DISTRIBUTION OF AN ORAL DOSE OF IMIDACLOPRID IN MALE RAT TISSUES', EKB Journal Management System, 46(5), pp. 1387-1394. doi: 10.21608/zjar.2019.48158
Nasr, H., Shalaby, A., Ragheb, D., Hendawi, M., Sauvé, S. ENZYME ACTIVITY AND TIME-COURSE QUANTITATIVE DISTRIBUTION OF AN ORAL DOSE OF IMIDACLOPRID IN MALE RAT TISSUES. EKB Journal Management System, 2019; 46(5): 1387-1394. doi: 10.21608/zjar.2019.48158

ENZYME ACTIVITY AND TIME-COURSE QUANTITATIVE DISTRIBUTION OF AN ORAL DOSE OF IMIDACLOPRID IN MALE RAT TISSUES

Zagazig Journal of Agricultural Research
Article 4, Volume 46, Issue 5, September 2019, Page 1387-1394  XML PDF (241.02 K)
Document Type: Original Article
DOI: 10.21608/zjar.2019.48158
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Authors
Heba E. Nasr email 1; A. A. Shalaby1; D. A. Ragheb1; M. Y. Hendawi1; Sébastien Sauvé2
1Plant Prot. Dept., Fac. Agric., Zagazig Univ., Egypt
2Chem. Dept., Univ. de Montréal, Canada
Abstract
The purpose of this study was to investigate the time course of changes in enzymes activity during distribution of imidaclopride (IMI) in Male Albino rat (Rattus norvegicus)  after 1, 3, 6, 12, 24 and 48 hr., treatment with a single oral dose (20 mg/kg body weight). The results showed significant gradual increase in liver and kidney function parameters coincided with accumulation of imidacloprid with varying quantities according to each organ, then gradually decreased to the end of study. The highest activity in liver enzymes, ALT (97.33 U/L), AST (78 U/L) ALP (169.66 IU/L) and LDH (5190.67 U/L) was recorded six hours post treatment, as well as increasing in total protein (8.94 g/dl). Kidney function parameters, i.e. urea, creatinine and uric acid levels (75.67, 2.283, 19.15 mg/dl, respectively) were also revealed the highest increase comparing with control. Liver and brain acetylcholinesterase (AChE) activity was reached the lowest level with the inhibition percent 82.9, 81.4%, respectively at the same time agreed with the concentration peak of IMI in blood (8.04 µg/ml) liver (2.42 µg/g) and brain (4.06 µg/g).The trend of maximal concentration of IMI in different organs and body fluids at six hours post dosing was in the following order: urine > blood > lungs > brain > testes > spleen > kidney > liver > muscles.
Keywords
Imidacloprid; time-course; residues; Enzymes; QuEChERS; male rats
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