Production of Vitamin B12 and Folate Using a Potent Mutant Strain of Klebsiella pneumonia | ||||
| Egyptian Journal of Chemistry | ||||
| Article 9, Volume 61, Issue 1, January and February 2018, Page 93-100 PDF (602.39 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2018.1629.1140 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Ibrahim Shabaan Abdelsalam 
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| Natural and microbial products Chemistry department, Drug and Pharmaceutical Division, National research Center | ||||
| Abstract | ||||
| Abstract The production of vitamin B12 and folate under solid state fermentation was investigated by using different mutants of klebsiella penumonia. The results showed that the isolate no ( EM33TE) produce the maximum B12 and folate yields ( 77.45 and 88.22 ug/ml) respectively. The supplementation of the fermentation medium by different carbon sources (glucose, fructose, mannose, sucrose, maltose, lactose ,starch and dextrin) indicated that the best carbon source was glucose and the maximum B12 yield (356.9ug/ml) was obtained by the sequential addition of glucose(10g ) to the fermentation medium . PCR studies revealed that clear differences at bands number and size between the original strain and its mutants using RAPD analysis by three different random primers, these differences in RAPD profiles confirmed the evidence of genetic variations of mutants and K.pneumonia genome after EMS-mutagenesis | ||||
| Keywords | ||||
| Keywords: Vitamin B12; folate; EMS-mutagenesis; Solid state fermentation; RAPD analysis | ||||
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