Bioguided screening for cytotoxic active constituents of Cuminum cyminum volatile oil
(2018). Bioguided screening for cytotoxic active constituents of Cuminum cyminum volatile oil. EKB Journal Management System, 27(2), 1-7. doi: 10.21608/zjps.2018.48350
. "Bioguided screening for cytotoxic active constituents of Cuminum cyminum volatile oil". EKB Journal Management System, 27, 2, 2018, 1-7. doi: 10.21608/zjps.2018.48350
(2018). 'Bioguided screening for cytotoxic active constituents of Cuminum cyminum volatile oil', EKB Journal Management System, 27(2), pp. 1-7. doi: 10.21608/zjps.2018.48350
Bioguided screening for cytotoxic active constituents of Cuminum cyminum volatile oil. EKB Journal Management System, 2018; 27(2): 1-7. doi: 10.21608/zjps.2018.48350

Bioguided screening for cytotoxic active constituents of Cuminum cyminum volatile oil

Zagazig Journal of Pharmaceutical Sciences
Article 1, Volume 27, Issue 2, December 2018, Page 1-7  XML PDF (212.37 K)
Document Type: Original Article
DOI: 10.21608/zjps.2018.48350
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Abstract
Volatile oils hold tremendous potential for the production of high quality plant based medicines. The aim of this study was to evaluate the cytotoxic activity of cumin volatile oil (CVO) against hepatocellular carcinoma (Hep-G2). In MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, CVO showed potent cytotoxic activity against Hep-G2 cell line with IC50=2.4 µg/ml. Bioguided fractionation of  CVO using silica  gel column chromatography afforded two active fractions(VO-1and VO-2)  with IC50= 0.6 and 0.9 µg/ml, respectively. GC-MS analysis was carried out for CVO and its two active fractions.  Crystallization of active fraction VO-1 lead to the isolation of cuminic acid with IC50=6 µg/ml. The structure of cuminic acid was established using IR, MS, 1H-NMR and 13C-NMR
Keywords
Cumin volatile oil; GC-MS; hepatocellular carcinoma (Hep-G2)
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