GENETIC AND MOLECULAR ANALYSES OF SOME COWPEA (Vigna unguicualata walp.) GENOTYPES SELECTED FOR BEAN BEETLS RESISTANCE | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 5, Volume 6, Issue 11, November 2015, Page 509-528 PDF (1.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2015.48465 | ||||
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| Authors | ||||
| A.S. Abd El-Shafy1; H.O. Sakr1; E.M. Zayed2 | ||||
| 1Forage Crops Research Department, FCRI, ARC, Giza - 12619, Egypt | ||||
| 2cell Research department, FCRI, ARC, Giza , Egypt | ||||
| Abstract | ||||
| The main objective of this investigation was to develop a genotype resistance of cowpea for bean beetls and high yield. Genetic parameters for yield and its component in five genotypes selected from five cultivars of cowpea. RAPD technique was used to confirm the genetic distance among genotypes. Significant and highly significant differences were found between the studied genotypes for all studied traits. The significance of mean squares of genotypes is an indicator for the presence of genetic variation among these genotypes. The mean performance showed that, the cowpea genotype selected (103) show the highest value for green fodder yield per plant (GFY/P) in the first and second cut, dry fodder yield per plant (DFY/P) in the second cut, crude protein (CP%) and digestible protein (DP/P%) in the second cut with the means of 502.0, 339.0, 86.67 GM /p, 19.54, and 15.19 %, respectively. The results revealed that the genotypic variance (VG) relative to environmental variation (VE) was large in magnitude for all traits except for crude protein (CP %) and digestible protein (DP %) in the second cut. The differences between genotypic coefficient of variability (GCV) and phenotypic coefficient of variability (PCV) were low, suggesting lower effects of environments for these traits. The estimated values of heritability in broad sense for all studied traits ranged from 36.96 to 98.23 % for digestible crude protein in the second cut to green fodder yield (GFY/P) in the first cut, respectively. The estimates of expected genetic advanced values for green fodder yield at first and second cut, dry fodder yield at the first and second cut, crude protein at the first and second cut, digestible crude protein in the first and second cut, shoot number, 50% flowering, 100 seed weight, and seed yield per plant are 89.16, 88.39, 83.21, 88.23, 6.76 4.21, 8.23, 5.23, 67.24, 33.2, 52.63, and 59.5 % respectively. This indicated that both additive as well as dominant gene action might involve in controlling these traits. It could be concluded that selection in advanced generations would be used to improve these traits. The pattern produced by ten primers showed a maximum number of 77 DNA bands ranging between 120 to 1050 bp. The primers, OP-C12, OP-C19, OP AX19, OPB11 and OP-B01 gave maximum number of polymorphic bands. All results are in favor at producing promising genotypes resistance to been beetles | ||||
| Keywords | ||||
| Cowpea; Genetic variance; Molecular analyses; Lesser bean beetls | ||||
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