Effects of Jasmonic and Salicylic Acids on Cell Division and Cell Cycle Progression | ||||
| Egyptian Journal of Botany | ||||
| Article 2, Volume 54, Issue 2, December 2014, Page 185-201 PDF (1.73 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2014.487 | ||||
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| Abstract | ||||
| THIS STUDY is concerned with the effect of both salicylic and jasmonic acids on mitotic cell division of Allium cepa (Lilliaceae) root tips and their ability to induce chromosomal aberrations. The study also deals with the use of image cytometry and SDS-PAGE techniques to estimate the changes in the cell cycle progression and seed protein banding pattern, respectively. Root tips of Allium cepa (Lilliaceae) treated for 3, 6 and 24 hr. At the cytological level both hormones caused reduction in mitotic index, particularly in roots treated with higher concentrations. A wide range of chromosomal abnormalities such as stickiness, c-metaphase, bridges, chromosome breaks, micronuclei and polyploidy cells were recorded in roots treated with both hormones. The effect of salicylic and jasmonic acids on DNA content corresponding to the proportion of cell cycle phases were calculated. Cytophotometric analysis showed decrease in the proportion of cells with 2C value (G1 phase) and increase in the fraction of cells in S and G2 phases as they compared with control. The lowest concentration of jasmonic acid in root treated for 24 hr and the lowest concentration of salicylic acid in root treated for 3 or 24 hr contradict this result since they increase the number of cells at G1 phase. The effect of both hormones seems to work on G2/M checkpoint at higher concentrations. At the biochemical level jasmonic acid induces changes in the electrophoretic profiles of seed protein of Vicia faba (Fabaceae). These changes include the absence of some bands and the appearance | ||||
| Keywords | ||||
| Allium; Vicia; Mitotic index; chromosomal aberrations; growth regulators; Image cytometry; SDS-PAGE | ||||
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