Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique
Elsayed, A., Badawy, A., Abdel-Aziz, A., Sarhan, M. (2019). Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique. EKB Journal Management System, 11(2), 15-23. doi: 10.21608/eajbse.2019.48766
Ayman N. Elsayed; Ahmed I.I. Badawy; Ahmed Abdel-Aziz; Moustafa Sarhan. "Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique". EKB Journal Management System, 11, 2, 2019, 15-23. doi: 10.21608/eajbse.2019.48766
Elsayed, A., Badawy, A., Abdel-Aziz, A., Sarhan, M. (2019). 'Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique', EKB Journal Management System, 11(2), pp. 15-23. doi: 10.21608/eajbse.2019.48766
Elsayed, A., Badawy, A., Abdel-Aziz, A., Sarhan, M. Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique. EKB Journal Management System, 2019; 11(2): 15-23. doi: 10.21608/eajbse.2019.48766

Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Article 2, Volume 11, Issue 2, December 2019, Page 15-23  XML PDF (916.47 K)
Document Type: Original Article
DOI: 10.21608/eajbse.2019.48766
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Authors
Ayman N. Elsayed1; Ahmed I.I. Badawy2; Ahmed Abdel-Aziz3; Moustafa Sarhan4
1Zoology Department, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt
2Parasitology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig City 44511, Sharkia, Egypt.
3Zoology Department, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt.
4Molecular Biology Lab., Zoology Department, Faculty of Science, Al Azhar University, Assiut City 71524, Assiut, Egypt.
Abstract
Molecular variability among female morphotypes (smooth, knobbed and linguiform B) and male of Haemonchus longistipes from Camelus dromedarius were analyzed by means of random amplified polymorphic DNA (RAPD) using 5 selected decamer primers. Results showed that primers OPA-04 and OPC-1 showed monomorphic bands for female morphotypes and male worms. In contrast, primers OPB-03, OPB-18, and OPD-05 yielded polymorphic bands among female morphotypes and male worms. A number of bands amplified by each primer varied from two to ten bands and ranged from 200 to 1750 bp. Band frequency per H. longistipes worm and per primer was estimated. The neighbor-joining clustering data based on pairwise distances among female morphotypes and male indicated that each sample had its own genetic peculiarity.
Keywords
Haemonchus longistipes; morphotypes; Camelus dromedarius; random amplified polymorphic DNA (RAPD); decamer primer
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