Expression of OCT4 Stem Cell Marker in Benign Prostatic Hyperplasia and Normal Tissue Around the Prostatic Carcinoma in a Sample of Iraqi Patients
noel, K., ibraheem, M., ahmed, B., hameed, A., khamees, N., akkila, S. (2020). Expression of OCT4 Stem Cell Marker in Benign Prostatic Hyperplasia and Normal Tissue Around the Prostatic Carcinoma in a Sample of Iraqi Patients. EKB Journal Management System, 43(1), 245-254. doi: 10.21608/ejh.2019.13791.1130
khalida noel; mustafa mohamed ibraheem; basim shehab ahmed; ahmed fakhir hameed; nibras hatim khamees; sameh samir akkila. "Expression of OCT4 Stem Cell Marker in Benign Prostatic Hyperplasia and Normal Tissue Around the Prostatic Carcinoma in a Sample of Iraqi Patients". EKB Journal Management System, 43, 1, 2020, 245-254. doi: 10.21608/ejh.2019.13791.1130
noel, K., ibraheem, M., ahmed, B., hameed, A., khamees, N., akkila, S. (2020). 'Expression of OCT4 Stem Cell Marker in Benign Prostatic Hyperplasia and Normal Tissue Around the Prostatic Carcinoma in a Sample of Iraqi Patients', EKB Journal Management System, 43(1), pp. 245-254. doi: 10.21608/ejh.2019.13791.1130
noel, K., ibraheem, M., ahmed, B., hameed, A., khamees, N., akkila, S. Expression of OCT4 Stem Cell Marker in Benign Prostatic Hyperplasia and Normal Tissue Around the Prostatic Carcinoma in a Sample of Iraqi Patients. EKB Journal Management System, 2020; 43(1): 245-254. doi: 10.21608/ejh.2019.13791.1130

Expression of OCT4 Stem Cell Marker in Benign Prostatic Hyperplasia and Normal Tissue Around the Prostatic Carcinoma in a Sample of Iraqi Patients

Egyptian Journal of Histology
Article 17, Volume 43, Issue 1, March 2020, Page 245-254  XML PDF (1 MB)
Document Type: Original Article
DOI: 10.21608/ejh.2019.13791.1130
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Authors
khalida noel email orcid 1; mustafa mohamed ibraheemorcid 1; basim shehab ahmedorcid 2; ahmed fakhir hameedorcid 1; nibras hatim khameesorcid 1; sameh samir akkilaorcid 1
1human anatomy departement/college of medicine/mustansiriyah university/baghdad/iraq
2pathology departement/college of medicine/mustansiriyah university/baghdad/iraq
Abstract
Objective: Prostatic diseases (benign and malignant) are broadly widespread in the world. Benign prostatic hyperplasia is a chronic entity reflected by enlarged prostatic tissue, triggering inferior urinary tract complaints. On the other hand, prostate cancer, is the second most common cancer in men and the fourth utmost commonly happening cancer generally. OCT4 referred to as octamer binding transcription factor 4, also recognized as POU5F1 (POU domain class 5 transcription factor 1), is a protein that in humans is coded by the POU5F1 gene. This protein is analytically elaborate in the self-renewal of undifferentiated embryonic stem cells. As such, our work is designed to evaluate the immunohistochemical examination of OCT4 expression in the prostatic epithelium in cases of benign prostatic hyperplasia (BPH) and in the epithelium of prostatic adenocarcinoma microenvironment (NPCA).  Patients and Methods: The prostate samples were acquired from 50 BPH patients, and 50 prostatic cancer patients. The samples were managed for immunohistochemical examination of OCT4 expression.   Results: Statistical analysis revealed a significant difference in the staining percentage between the BPH and NPCA group (P-value=0.009), and there was significant staining expression of OCT4 in NPCA group as compared to BPH group (P-value=0.000). Also, there was a significant elevation of the total score of OCT4 in NPCA group (P-value=0.036) as compared with BPH group.  Conclusions: OCT4 is over expressed in normal tissue around prostatic carcinoma as compared to benign tissue in BPH, and thus OCT4 can be used as a stem cell marker for prostatic tissue tuomorigenicity.
Keywords
Keywords: immunohistochemistry; OCT4; prostatic cancer; BPH
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