Hepatoprotective Effect of Coumarin and Chlorophyll Against Aflatoxicosis in Rat | ||||
| Journal of Animal and Poultry Production | ||||
| Article 6, Volume 7, Issue 12, December 2016, Page 483-490 PDF (352.63 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2016.48813 | ||||
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| Authors | ||||
| M. S. Abdel-Latif1; K. M. Elmeleigy2; Marwa M. S. Khattab3; Sara M. Mohamd2 | ||||
| 1Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt. | ||||
| 2Regional Center For Food and Feed, Agricultural Research Center, Ministry of Agriculture, Giza, Egypt. | ||||
| 3Department of Pathology, Fac. of Veterinary Medicine, Cairo University, Giza Square, Giza, Egypt. Postal code 12211 | ||||
| Abstract | ||||
| Aflatoxin contamination of animal diet is still a major problem for breeders since it hasadverse effects on animal health and productivity. This study was carried out to investigate the effect of using coumarin and/or chlorophyll in rat diet against aflatoxicosis. Fifty four rats were randomly assigned into 7 groups ''6 rats each'' and group 5 ''12 rats''. Group 1 was negative control. Group 2 received water with coumarin 0.5%. Group 3 received water with chlorophyll 0.5%. Group 4 received water with coumarin 0.5% and chlorophyll 0.5%. Group 5-8 received basal diet with aflatoxin B1 1000 ppb. Group 6-8 were administered similar treatments as groups 2-4. The experiment ended after 8 weeks. Water and feed consumption were measured and feed conversion rate were calculated. Random glucose level, total lipid, total cholesterol, triglyceride, total protein, ALT , AST, creatinine and urea were determined in serum. Histopathological examination of liver, kidney and pancreas was performed. The conversion rate was significantly low in group 5 compared to groups 6-8. The serum glucose, cholesterol, AST and ALT were elevated in group 5 compared to groups 6-8. The liver lesions observed in group 5 represented in vacuolation and necrosis were alleviated in groups 6-8. The necrosis and inflammatory cells infiltration in pancreas of group 5 were absent in groups 6-8. In conclusion, the coumarin and or chlorophyll possessed a hepatoprotective effect against aflatoxincosis. | ||||
| Keywords | ||||
| Aflatoxin; Coumarin; Chlorophyll; Histopathological examination | ||||
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