Metabolic Resistance Mechanisms of Different Pesticides in The Two-Spotted Spider Mite, Tetranychus urticae Koch (Tetranychidae:Acari) | ||||
Acarines: Journal of the Egyptian Society of Acarology | ||||
Article 8, Volume 3, Issue 1, January 2009, Page 45-53 PDF (175.68 K) | ||||
Document Type: Original research articles | ||||
DOI: 10.21608/ajesa.2009.4965 | ||||
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Authors | ||||
Salwa Negm1; A. Saleh1; A. Abd El-Hady1; H. Bekheat2; Rania Abd El-Wahab2 | ||||
1Pesticide Dept., Faculty of Agriculture ,Mansoura Univ. | ||||
2Plant Protection Research Institute,Dokki,Giza, | ||||
Abstract | ||||
An investigation was performed to determine the possible role of detoxification metabolism in resistant strains of Tetranychus urticea Koch.Adult females were subjected to selection pressure through 40 generations by LC50's and LC90's of Vertimec (Avermectines), Cypermethrin (Pyrethroids), Methomyl (Carbamates) and Malathion (O.P), were (41.308, 42.021, 44.881, and 49.265 folds) and (49.815 ,55.411 ,63.972 ,and 90.00 folds), respectively, whereas RRS slopes ranged from 0.884 to 1.395 folds. Comparative assay with esterases (EST) and mixed function oxidases(MFO) showed that LC50's of Mal-Resistant strains had higher EST. activiy (4.71) with lower MFO activity (3.80),while ,LC50's of Vert-Rresistant strains had lower EST. activiy (2.70) with higher MFO activity (4.26). Synergist experiments showed low synergism by S,S,S tributylphosphorotrithioate (DEF) of EST activiy ranged from 4.81 to 2.99 folds,while synergism by Piperonyl Butoxide (PBO) of MFO activity ranged from 4.27 to 3.82folds to LC50's pesticides resistant strains. | ||||
Keywords | ||||
Tetranychus urticae; resistance; Selection Pressure; Avermectines; Pyrthroids; Carbamates; OP's; Mixed Function Oxidases; Esterases; PBO; def | ||||
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