PROTECTIVE EFFECTS OF GRAPE SEED OIL AGAINST CCl4 INDUCED OXIDATIVE STRESS IN RAT BRAIN | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 5, Issue 12, December 2014, Page 281-303 PDF (747.37 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2014.50047 | ||||
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| Authors | ||||
| A.F.M. Ismail1; M.M.T. Eassawy2; A.A.M. Salem2 | ||||
| 1Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT).Nasr City, Cairo, Egypt. | ||||
| 2Regional Center for Food and Feed (RCFF), Agriculture Research Center, Giza, Egypt. | ||||
| Abstract | ||||
| This study aimed to investigate the possible beneficial effects of grape seed oil (GSO) on CCl4-induced acute brain toxicity in rats. The animals injected with acute dose of CCl4 (2 ml / kg b. wt.) showed a statistical significant decrease in the blood hematological parameters (WBCs, RBCs, PLT counts, Hb and MCV values), and a significant elevation in serum TNF-α, and IL-6 levels. In addition, damage of the brain DNA, disturbance of the brain antioxidant status; a significant decrease in SOD, GSH-Px and Catalase (CAT) activities as well as GSH level, accompanied with a significant elevation in MDA and NO levels, as well as a high significant elevation in xanthine oxidase and inducible nitric oxide synthase (iNOS) gene expressions have been observed due to the oxidative stress produced after CCl4 injection. The pretreatment of GSO exert significant ameliorated the hematologic parameters, and serum TNF-α and IL-6 levels, protected DNA damage, improved SOD, GSH-Px and CAT activities as well as GSH, MDA and NO levels and down-regulation of xanthine oxidase (XO) and inducible nitric oxide synthase (iNOS)gene expression levels in the brain tissues of CCl4 injected rats. These findings suggest that GSO prevents acute brain damage due to CCl4 toxicity, which could be attributed to its immuno-modulation and antithrombotic, antiapototic, antioxidant, and anti-inflammatory activities. It can be suggested that GSO which containing high level of polyphenolic compounds, essential fatty acids and vitamin E (Tocopherol) revealed to protect the brain from CCl4 toxicity and/or any other toxicant cause oxidative stress. | ||||
| Keywords | ||||
| Grape Seed Oil (GSO); carbon tetrachloride (CCl4); brain damage; antioxidants; Cytokines; Xanthine oxidase; inducible nitric oxide synthase (iNOS) relative gene expression | ||||
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