COMBINING ABILITY IN MAIZE UNDER TWO NITROGEN LEVELS AND ASSESSING GENETIC DIVERSITY USING RAPD MARKER | ||||
| Journal of Plant Production | ||||
| Article 9, Volume 6, Issue 12, December 2015, Page 2051-2067 PDF (646.95 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2015.52432 | ||||
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| Authors | ||||
| M. M. Kamara1; M. R. Rehan2 | ||||
| 1Agronomy Dep., Faculty of Agriculture 33516, Kafrelsheikh University, Kafr El-Sheikh, Egypt. | ||||
| 2Genetics Dep., Faculty of Agriculture 33516, Kafrelsheikh University, Kafr El-Sheikh, Egypt. | ||||
| Abstract | ||||
| A half diallel cross among seven white maize inbred lines was made in 2012 growing season. The resulted 21 F1 crosses and the commercial check hybrid SC10 were evaluated under two different nitrogen levels, i.e. 80 and 120 kg N fad-1 at the Experimental Farm, Faculty of Agriculture, Kafrelsheikh University in 2013 growing season, to estimate general and specific combining ability effects (GCA and SCA) and their interactions with nitrogen levels as well as identify the superior inbred lines and crosses. Data were collected for number of days to 50% silking, ear length, ear diameter, number of rows ear-1, number of kernels row-1 and grain yield plant-1 and were analyzed according to Griffing (1956) method-4 model-1(fixed model). The results revealed that, the mean squares due to nitrogen levels (N), genotypes (G), crosses (Cr.), G × N interaction and Cr. × N interaction were significant for all the studied traits. General and specific combining ability (GCA and SCA) mean squares were significant for all the studied traits under the two nitrogen levels and their combined data. Both GCA and SCA effects were significantly interacted with nitrogen levels for most of the studied traits. The non-additive gene action played an important role in the inheritance of all the studied traits, except days to 50% silking and grain yield plant-1under the two nitrogen levels and their combined data. The inbred lines P5, P6 and P7 showed the best desirable GCA effects for earliness, whereas P1, P2 and P4 were the best general combiners for grain yield plant-1. The best crosses showed desirable SCA effects were P3×P5, P3×P7, P5×P6 and P6×P7 for earliness and P1×P4, P2×P4, P3×P6 and P5×P7 for grain yield plant -1 under the two nitrogen levels and the combined data. Two crosses P1×P4 and P2×P4 gave significantly positive superiority in grain yield over the check hybrid SC10 under the two nitrogen levels and the combined data. The genetic diversity (GD) among the seven parental inbred lines was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Seven random primers were used to give a total 70 reproducible RAPD fragments, of them 56 (77.88%) being polymorphic. The GD among the inbred lines differed from 0.333 to 0.655 with an average of 0.503. The estimate value of correlation coefficient between GD and mean performance of the F1 hybrids for grain yield plant-1 was low (r = 0.335) or not high enough to be of predictive value. Therefore, the RAPD marker could not be predicted about the mean performance of the grain yield plant-1 in this study. | ||||
| Keywords | ||||
| Zea mays L; Inbred Lines; GCA; SCA; nitrogen levels; RAPD; Genetic diversity | ||||
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