Differential Hepatic Gene Expression and Antioxidant Activity in Male and Female Rats Induced by Subchronic Aflatoxicosis B1
Omran, G., Abo El-Maali, N., Ismail, M., Ahmed, N., Mostafa, M., Nasser, N. (2019). Differential Hepatic Gene Expression and Antioxidant Activity in Male and Female Rats Induced by Subchronic Aflatoxicosis B1. EKB Journal Management System, 27(2), 13-28. doi: 10.21608/mjfmct.2019.52468
Ghada Ali Omran; Nagwa Thabet Abo El-Maali; Mady Ahmed Ismail; Nashwa Ahmed; Mohamad Mostafa; Nasser Masood Nasser. "Differential Hepatic Gene Expression and Antioxidant Activity in Male and Female Rats Induced by Subchronic Aflatoxicosis B1". EKB Journal Management System, 27, 2, 2019, 13-28. doi: 10.21608/mjfmct.2019.52468
Omran, G., Abo El-Maali, N., Ismail, M., Ahmed, N., Mostafa, M., Nasser, N. (2019). 'Differential Hepatic Gene Expression and Antioxidant Activity in Male and Female Rats Induced by Subchronic Aflatoxicosis B1', EKB Journal Management System, 27(2), pp. 13-28. doi: 10.21608/mjfmct.2019.52468
Omran, G., Abo El-Maali, N., Ismail, M., Ahmed, N., Mostafa, M., Nasser, N. Differential Hepatic Gene Expression and Antioxidant Activity in Male and Female Rats Induced by Subchronic Aflatoxicosis B1. EKB Journal Management System, 2019; 27(2): 13-28. doi: 10.21608/mjfmct.2019.52468

Differential Hepatic Gene Expression and Antioxidant Activity in Male and Female Rats Induced by Subchronic Aflatoxicosis B1

Mansoura Journal of Forensic Medicine and Clinical Toxicology
Article 2, Volume 27, Issue 2, July 2019, Page 13-28  XML PDF (1022.38 K)
Document Type: Original Article
DOI: 10.21608/mjfmct.2019.52468
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Authors
Ghada Ali Omran1; Nagwa Thabet Abo El-Maali2; Mady Ahmed Ismail3; Nashwa Ahmed4; Mohamad Mostafa5; Nasser Masood Nasser6
1Forensic Medicine & Clinical Toxicology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
2Chemistry Department, Faculty of Science, Assiut University, Assiut, Egypt.
3Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt.
4Forensic Medicine & Clinical Toxicology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
5Histology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
6Chemistry Department, Faculty of Education and Sciences, Aden University, Aden, Yemen.
Abstract
Aflatoxin B1 (AFB1) is the most toxic mycotoxin that was proven to be deleterious to human and several animal species. Current work aimed at evaluating the sex-based differential hepatic genotoxic effect and the antioxidant activity as implications of subchronic aflatoxicosis B1. Albino rats were used that comprised two equal AFB1treated groups with AFB1 contaminated olive oil (50µg/kg) and a control group for each gender that received the vehicle only. Parts of animals’ livers were homogenized for gene expression assessment using quantitative RT-PCR and antioxidant activity analyses. Caspase-3 immunohistopathological examination was concomitantly undertaken. Results showed that AFB1 induced significant overexpression in cell cycle proliferation (ODC1), apoptosis (Aen), and antioxidant heme oxygenase (Hmox) genes in males alongside Bax (Bcl2- associated protein) under-expression. Meanwhile, female rats showed significant overexpression for (Hmox) and under-expression of Bax and Tnf (Tumor necrosis factor). Concomitant total hepatic antioxidant activity of liver homogenates showed a reduction in males, contrasting females. Degenerate vacuolated hepatocytes, polymorphic nuclei, cellular infiltration with concomitant Caspase-3 positive cells were profound findings in male rats. Hence, AFB1 is deferentially genotoxic at the given dose especially to male rats towards carcinogenicity, oxidative stress and apoptosis compared to a brief but compensated oxidative stress in females.
Keywords
genotoxicity; Aflatoxin B1; hepatic; Rats' gender
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