Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231
DH, S., MA, H. (2017). Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231. EKB Journal Management System, 2(1), 26-39. doi: 10.21608/mb.2017.5255
Sheir DH; Hafez MA. "Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231". EKB Journal Management System, 2, 1, 2017, 26-39. doi: 10.21608/mb.2017.5255
DH, S., MA, H. (2017). 'Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231', EKB Journal Management System, 2(1), pp. 26-39. doi: 10.21608/mb.2017.5255
DH, S., MA, H. Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231. EKB Journal Management System, 2017; 2(1): 26-39. doi: 10.21608/mb.2017.5255

Antibiofilm activity of Streptomyces toxytricini Fz94 against Candida albicans ATCC 10231

Microbial Biosystems
Article 4, Volume 2, Issue 1, June 2017, Page 26-39  XML PDF (461.13 K)
Document Type: Original Article
DOI: 10.21608/mb.2017.5255
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Authors
Sheir DH email orcid 1; Hafez MA2
1Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Division, National Research Centre, Cairo,
2Department of Botany and Microbiology, Faculty of Science, Al Azhar University, Cairo, 11651, Egypt
Abstract
Candida albicans is a significant cause of morbidity and mortality in immunocompromised patients worldwide. Biofilm formation by Candida species is a significant virulence factor for disease pathogenesis. Keeping in view the importance of Streptomyces' metabolites, the present study was initiated during the bioprospecting programme of Egyptian Streptomyces carried by the authors since 2013. Native Streptomyces isolates were recovered from soil samples collected from different governorates. Antifungal activity of forty isolates of Streptomyces were performed against planktonic (free cells) of C. albicans ATCC 10231 and resistant clinical Candida isolates. Streptomyces isolates showed high inhibition activity against free cells of Candida were further assayed against biofilm of C. albicans reference strain. The most active Streptomyces sp. (no.6) was identified phenotypically, biochemically and by using 16S rRNA. The 16S rRNA sequences obtained were compared with those deposited in the GenBank Database and registered with accession number KM052378 as S. toxytricini Fz94. Screening of S. toxytricini Fz94 extract capability in prevention and destruction of C. albicans reference strain biolfilm was assessed by resazurin dye adopted technique. In the pre-exposure scheme, the lowest concentration of 5 gL-1 showed biofilm viability inhibition of 92% after 120 min, while Ketoconazole® gave 90 % inhibition at concentration of 2 gL-1. In post exposure, the concentration of S. toxytricini Fz94 extract 7gL-1 caused 82 % inhibition of biofilms viability after 120 min, while Ketoconazole did not show any destruction capability. The cytotoxicity of S. toxytricini Fz94 crude extract results showed that it was nontoxic at 10 gL-1. S. toxytricini Fz94 is maintained in the Fungarium of Arab Society for Fungal Conservation (ASFC) with accession number FSCU-2017-1110.
Keywords
Bioprospecting; Egypt; Ketoconazole; Streptomyces toxytricini Fz94; resazurin
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