IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES
Attaya, A., El-Sarag, E., Mekki, B., Ibrahim, A. (2017). IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES. EKB Journal Management System, 44(4), 1203-1215. doi: 10.21608/zjar.2017.52912
Ahmed S. Attaya; Eman I. El-Sarag; B- E. Mekki; Aya E-E. Ibrahim. "IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES". EKB Journal Management System, 44, 4, 2017, 1203-1215. doi: 10.21608/zjar.2017.52912
Attaya, A., El-Sarag, E., Mekki, B., Ibrahim, A. (2017). 'IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES', EKB Journal Management System, 44(4), pp. 1203-1215. doi: 10.21608/zjar.2017.52912
Attaya, A., El-Sarag, E., Mekki, B., Ibrahim, A. IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES. EKB Journal Management System, 2017; 44(4): 1203-1215. doi: 10.21608/zjar.2017.52912

IN VITRO REGULATION AND ENHANCEMENT OF ORGANOGENESIS IN SIX CANOLA (Brassica napus, L.) GENOTYPES

Zagazig Journal of Agricultural Research
Article 2, Volume 44, Issue 4, July 2017, Page 1203-1215  XML PDF (306.59 K)
Document Type: Original Article
DOI: 10.21608/zjar.2017.52912
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Authors
Ahmed S. Attaya email 1; Eman I. El-Sarag2; B- E. Mekki3; Aya E-E. Ibrahim2
1Plant Prod. Dep., Fac. Environ. Agric. Sci., Arish Univ., North Sinai, Egypt
2Plant Prod. Dept., Fac. Environ. Agric. Sci., Arish Univ., North Sinai, Egypt
3Field Crops Res. Dept., Nat. Res. Cent., Giza, Egypt
Abstract
This study was designed to investigate the effect of some factors affecting on in vitro propagation of six canola genotypes (Serw-6, Pactol, Wan-you 25, RG-4514, Serw-4 and AD-201/Gi/ 51). During establishment stage, different medium types (MS and B5) and cytokinin types (BA, Kin and 2ip) were investigated. MS medium supplemented with BA at 1.0 mgl-1 proved to be the best treatment for all tested genotypes during this stage. Also, different explant types (shoot tip, internode and hypocotyl), BA concentrations (0.0, 1.0, 3.0 and 5.0 mg l-1) were examined during multiplication stage. Results indicated that while explant type had no effect on shoot induction and growth, BA proved to be the best cytokinin especially at 1.0 mgl-1 since it produced the maximum number of shoots/ explant for the most tested genotypes. During rooting stage, effects of MS medium strength (full and half) and auxin type (IAA, IBA and NAA) at the concentration of 1.0 mgl-1 were evaluated. Generally, using of full MS medium supplemented with 1.0 mgl-1 IBA stated to be the most suitable treatment for root induction and growth of the most investigated genotypes. Obtained plantlets were successfully acclimatized (60-80% survivability) in peat moss and sand (1:1, V/V) medium in the greenhouse.
Keywords
Brassica napus; Shoot induction and multiplication; Plant growth regulators (PGRs); media composition; Murashige and Skoog medium (MS); gamborg medium (B5); in vitro root formation
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