EFFECT OF ENDOXAN AND ETHYLE METHANE SULPHONATE ON CHROMOSOMAL BEHAVIOUR AND MITOTIC INDEX IN SOMATIC CELLS OF ONION ( Allium cepa ,L .) | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 4, Issue 3, March 2013, Page 93-102 PDF (606.45 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2013.53027 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A. M. El - Adl; Kawther S. Kash; K. A. Zaied; Mervat I. Kamal | ||||
| Dept. of Genetics , Fac. of Agric. , Mans. Univ. , Egypt. | ||||
| Abstract | ||||
| This study aimed to investigate abnormal chromosomal behaviour in mitosis resulted from Ethyle methane sulphonate ( EMS ) and Endoxan . Commercial onion bulbs were used as a material of mitotic cell division . In addition , this study compared between the effect of mutagenic agent ( EMS ) with anticancer compound (Endoxan ) on mitotic index . Onion bulbs were germinated under the effect of EMS and Endoxan , in addition to control treated with tap water . The differences between treatments and control were statistically evaluated . The results achieved that mitotic index of control had the largest number of normally divided cells about 15% , while Endoxan about 10% and EMS about 4% . This indicated that EMS had a strong effect of inhibiting mitosis than of Endoxan. In general , Endoxan and EMS reduced mitotic index than control in addition to several types of chromosomal aberrations . These chemical agents included sticky chromosomes , anaphase bridge chromosomes , lagging chromosomes , disrupted chromosome segregation and star cluster chromosomes . with variable percentage of each type. This reflects the dangerous of these chemical compounds on mitotic cell division . | ||||
| Keywords | ||||
| Allium cepa; L . chromosomal aberrations; cyclophosphamide; cytogenetic effects; Ethyle methane sulphonate; Mitotic index | ||||
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