The diagnostic roles of C4D expression in SOME AUTOIMMUNE BULLOUS DERMATOSES IN ZAGAZIG UNIVERSITY HOSPITALS; an immunohistochemical study
Mohammed Soliman, E., ElKashishy, K., Metwally, M., Hassan, A. (2021). The diagnostic roles of C4D expression in SOME AUTOIMMUNE BULLOUS DERMATOSES IN ZAGAZIG UNIVERSITY HOSPITALS; an immunohistochemical study. EKB Journal Management System, 27(5), 767-775. doi: 10.21608/zumj.2019.7305.1032
Ebtehal El Sayed Mohammed Soliman; Kamal Ahmed ElKashishy; Mohammed Ibrahim Metwally; Abeer Magdy Hassan. "The diagnostic roles of C4D expression in SOME AUTOIMMUNE BULLOUS DERMATOSES IN ZAGAZIG UNIVERSITY HOSPITALS; an immunohistochemical study". EKB Journal Management System, 27, 5, 2021, 767-775. doi: 10.21608/zumj.2019.7305.1032
Mohammed Soliman, E., ElKashishy, K., Metwally, M., Hassan, A. (2021). 'The diagnostic roles of C4D expression in SOME AUTOIMMUNE BULLOUS DERMATOSES IN ZAGAZIG UNIVERSITY HOSPITALS; an immunohistochemical study', EKB Journal Management System, 27(5), pp. 767-775. doi: 10.21608/zumj.2019.7305.1032
Mohammed Soliman, E., ElKashishy, K., Metwally, M., Hassan, A. The diagnostic roles of C4D expression in SOME AUTOIMMUNE BULLOUS DERMATOSES IN ZAGAZIG UNIVERSITY HOSPITALS; an immunohistochemical study. EKB Journal Management System, 2021; 27(5): 767-775. doi: 10.21608/zumj.2019.7305.1032

The diagnostic roles of C4D expression in SOME AUTOIMMUNE BULLOUS DERMATOSES IN ZAGAZIG UNIVERSITY HOSPITALS; an immunohistochemical study

Zagazig University Medical Journal
Article 260, Volume 27, Issue 5, September 2021, Page 767-775  XML PDF (333.87 K)
Document Type: Original Article
DOI: 10.21608/zumj.2019.7305.1032
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Authors
Ebtehal El Sayed Mohammed Soliman email 1; Kamal Ahmed ElKashishy2; Mohammed Ibrahim Metwally3; Abeer Magdy Hassan4
1Pathology Department,Faculty of Medicine , Zagazig University, Zagazig,Sharkeya , Egypt
2Pathology Department - Faculty of Medicine - Zagazig University -Zagazig - sharkeya - Egypt
3Dermatology Department - Faculty of Medicine - Zagazig University - Zagazig - Sharkeya - Egypt
4Pathology Department - Faculty of MEdicine - Zagazig University - Zagazig - Sharkeya - Egypty
Abstract
Background: Autoimmune bullous skin dermatoses (AIBD) diagnosis relies on direct immunofluorescence examination performed on frozen tissue sections. However, this is not always available for DIF; therefore, alternative techniques needed for diagnosis. We tested the usefulness of C4d immunohistochemistry on formalin-fixed, paraffin-embedded tissue (FFPE) sections for the diagnosis of AIBD.
Objective: to evaluate the role of C4d expression using immunohistochemistry in diagnosis of some autoimmune bullous skin diseases.
Methods: This study included FFPE tissue blocks of 35 cases from archives of pathology department, Faculty of medicine, Zagazig University in the period from January 2017 to December 2017. These 35 cases were diagnosed histopathologically as: 30 cases autoimmune bullous dermatoses (18 pemphigus vulgaris, 6 bullous pemphigoid, 3 pemphigus foliaceus and 3 drug induced pemphigus) and 5 cases erythema multiforme. Specimens were obtained as punch biopsy from the edge of a recent bullous lesion. C4d immunostaining was performed and correlated with clinicopathology.
Results: C4d immunohistochemistry was a reliable method for detecting AIBD in 29 of 30 cases diagnosed by histopathology, with 96.7% sensitivity. Also it was efficient in ruling out of the all 5 negative cases ruled out by histopathology with 100% specificity.
Conclusion: When correlated with the light microscopic and clinical findings, the C4d assay defines an important diagnostic adjunct in the evaluation of some autoimmune vesiculobullous dermatoses. It may prompt further DIF testing or, in some instances, may even define a reasonable substitute for DIF and/or add to the morphologic assessment of a biopsy specimen submitted for routine light microscopic assessment.
Keywords
C4d; Immunohistochemistry; Autoimmune Bullous Dermatoses
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