Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.
Abd El-kafie, O., El-Saka, M., Abd El-Baset, M., khalil, S. (2019). Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.. EKB Journal Management System, 10(7), 495-501. doi: 10.21608/jpp.2019.53543
Omaima M. Abd El-kafie; Magda M. El-Saka; M. M. Abd El-Baset; Sara M. M. E. I. khalil. "Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.". EKB Journal Management System, 10, 7, 2019, 495-501. doi: 10.21608/jpp.2019.53543
Abd El-kafie, O., El-Saka, M., Abd El-Baset, M., khalil, S. (2019). 'Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.', EKB Journal Management System, 10(7), pp. 495-501. doi: 10.21608/jpp.2019.53543
Abd El-kafie, O., El-Saka, M., Abd El-Baset, M., khalil, S. Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.. EKB Journal Management System, 2019; 10(7): 495-501. doi: 10.21608/jpp.2019.53543

Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.

Journal of Plant Production
Article 4, Volume 10, Issue 7, July 2019, Page 495-501  XML PDF (694.71 K)
Document Type: Original Article
DOI: 10.21608/jpp.2019.53543
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Authors
Omaima M. Abd El-kafie1; Magda M. El-Saka2; M. M. Abd El-Baset1; Sara M. M. E. I. khalil1
1Vegetable and Floriculture Dept, Fac. of Agric. Mansoura Univ.
2Ornamental Plants and Landscape Gardening Dept., Hort. Res. Inst., ARC
Abstract
The aim of this study is to improve the propagation of Impatiens balsamina, L by an efficient protocol for in vitro propagation was successfully established through tissue culture technique. Cotyledonary node with sections of cotyledon, section of cotyledons and hypocotyls were derived from 21 days in vitro germinating seeds after surface sterilization. They were cultured for shoot multiplication and callus induction on MS medium fortified with various concentrations of BAP, TDZ or combination between BAP and NAA. It was determined that cotyledonary node with sections of cotyledon on MS medium supplemented with 4.5 mg/L BAP for maximum shoots number (4.33shoots) and callus induction 3.78. The best shoot length (3.08 cm) within 8 weeks from regeneration was achieved on MS medium fortified with 1.0 mg/L BAP + 0.5 mg/L NAA. The number of shoots formed per explants increased by re-culturing for six weeks on the same medium (4.5 mg/L BAP alone), since it produced (9 shoots). While the best shoot length (4cm) was obtained by sub culturing the explants on MS medium received 1.0 mg/L BAP + 0.5 mg/L NAA to MS without growth regulators after six weeks. The best callus fresh weight (9.32 g) and callus volume (7.76 cm3) were recorded with using TDZ at 3.0 mg/L that sub cultured on medium fortified with 4.5 mg/L BAP. In addition the highest dry weight (0.81 g) was obtained by re - culturing on the same medium that contained with 4.5 mg/L BAP.
Keywords
tissue culture; balsam; auxin; Cytokinin; gibberellins; BAP; TDZ; IBA; NAA; Callus
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