SYMBIOTIC NITROGEN FIXATION OF FABA BEAN PLANTS INOCULATED WITH SALT-TOLERANT Rhizobium ISOLATES | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 2, Volume 3, Issue 10, October 2012, Page 411-423 PDF (424.44 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2012.55014 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nagwa M. M. El-Khateeb; M. E. Shalaby; E. B. A. Belal; SH. M. A. El-Gremi | ||||
| Dept. of Agric. Botany, Agric. Microbiology, Faculty of Agric., Kafrelsheikh Univ., Egypt | ||||
| Abstract | ||||
| Rhizobium leguminosarum biovar viciae isolated from salt-affected faba bean fields were in vitro tested. Different isolates were found to be varied in their tolerance to salinity. F1 and E1 were found to be the most salt-tolerant isolates even at EC-level of 12 ds m-1. Seeds of Nubaria 1 cv. were inoculated with either F1 or E1, in comparison with a less salt-tolerant one (F9) in pots their salinity were adjusted at 0, 3, 6, 9 and 12 ds m-1 under aseptic conditions. Number and dry weight of nodules were decreased strongly by increasing salinity levels in comparison with control. Significant enhancement of N % accumulated in the shoots was also noticed due to the biological inoculation with both Rhizobium isolates in presence or absence of salinity. Under non-aseptic conditions, efficacy of F1 and E1 with the effect of mineral N-supply (25 and 100 %) was compared. In addition to Nubaria 1 cv., Sakha 1 cv. was also used in the evaluation process for N-fixation at 0, 6, 9 ds m-1 levels. Number of nodules formed on Nubaria 1 at 9 ds m-1 was highly increased from 7.86 and 5.44 for 25 and 100 % N-supply to 82.33 and 93.33 for F1 and E1, respectively. Accordingly, nodular dry weights were also increased to 0.40 and 0.41 g plant-1 for F1 and E1 instead of 0.13 and 0.08 g plant-1 for 25 and 100 % N, respectively. The beneficial effects of F1 and E1 inoculation were also extended to enhance N % of shoots and seed yield parameters. Therefore, the symbiotic N2-fixation process has a great potential to improve growth and productivity of faba bean plants in comparison with the mineral N-supply to overcome the harmful effects of salinity. | ||||
| Keywords | ||||
| Vicia faba; Rhizobium; salinity; mineral N-supply | ||||
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