Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2 | ||||
Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
Article 6, Volume 11, Issue 1, December 2019, Page 47-57 PDF (874.74 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsg.2019.55269 | ||||
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Authors | ||||
omnia mohamed abdallah1; Khaled Z. EL-Baghdady2; Mostafa M.H. Khalil3; Mervat I. El Borhamy4; Gamal Meligi3 | ||||
1Department of Microbiology, Faculty of Dentistry, Misr International University, Cairo, Egypt | ||||
2Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
3Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
4-Department of Microbiology, Faculty of Pharmacy, Misr International University, Cairo, Egypt -International Medical Center, Clinical Microbiology Laboratory, Cairo, Egypt | ||||
Abstract | ||||
Green biosynthesis of selenium nanoparticles (SeNPs) using Bacillus tropicus Ism 2 (MK332444) isolated from soil polluted with industrial wastewater near the Ismailia Canal, Egypt, was carried out. SeNPs were biogenically synthesized using bacterial pellets mixed with 1 mM Na2SeO3. Characterization of biosynthesized SeNPs was carried out by UV-Vis spectrum where SeNPs solution exhibited characteristic peak at 270 nm. Dynamic Light Scattering (DLS) analysis and High-Resolution Transmission Electron Microscope (HRTEM) showed spherical shaped SeNPs with size ranged from 60 to 125 nm with PDI= 0.544 and zeta potential value of -30.6. Biologically synthesized SeNPs showed no antibacterial activity against both Gram-positive and Gram-negative bacteria. Cytotoxicity of SeNPs was studied against Huh-7 human liver cell line and showed low cytotoxicity with IC50 value of367±5.5 μg/ml. In conclusion in this work Bacillus tropicus Ism 2 locally isolated bacterium showed the capability to biosynthesize SeNPs with spherical shape and low cytotoxicity. | ||||
Keywords | ||||
Selenium nanoparticles; DLS; TEM; Cytotoxicity; Bacillus tropicus Ism 2 | ||||
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