Evaluation of Salt-Tolerant Azospirillum lipoferum and Its Role in Improvement of Wheat Growth Parameters
El-Akhdar, I., El-Sheekh, M., Allam, N., Kamal, F., Abou-Shanab, R., Staehelin, C. (2019). Evaluation of Salt-Tolerant Azospirillum lipoferum and Its Role in Improvement of Wheat Growth Parameters. EKB Journal Management System, 3(2019), 163-178. doi: 10.21608/jenvbs.2019.16428.1069
Ibrahim El-Akhdar; Mostafa El-Sheekh; Nanis G. Allam; Faiza Kamal; Reda Abou-Shanab; Christian Staehelin. "Evaluation of Salt-Tolerant Azospirillum lipoferum and Its Role in Improvement of Wheat Growth Parameters". EKB Journal Management System, 3, 2019, 2019, 163-178. doi: 10.21608/jenvbs.2019.16428.1069
El-Akhdar, I., El-Sheekh, M., Allam, N., Kamal, F., Abou-Shanab, R., Staehelin, C. (2019). 'Evaluation of Salt-Tolerant Azospirillum lipoferum and Its Role in Improvement of Wheat Growth Parameters', EKB Journal Management System, 3(2019), pp. 163-178. doi: 10.21608/jenvbs.2019.16428.1069
El-Akhdar, I., El-Sheekh, M., Allam, N., Kamal, F., Abou-Shanab, R., Staehelin, C. Evaluation of Salt-Tolerant Azospirillum lipoferum and Its Role in Improvement of Wheat Growth Parameters. EKB Journal Management System, 2019; 3(2019): 163-178. doi: 10.21608/jenvbs.2019.16428.1069

Evaluation of Salt-Tolerant Azospirillum lipoferum and Its Role in Improvement of Wheat Growth Parameters

Environment, Biodiversity and Soil Security
Article 11, Volume 3, Issue 2019, February 2019, Page 163-178  XML PDF (3.77 MB)
Document Type: Original Article
DOI: 10.21608/jenvbs.2019.16428.1069
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Authors
Ibrahim El-Akhdar email 1; Mostafa El-Sheekh2; Nanis G. Allam2; Faiza Kamal1; Reda Abou-Shanab3; Christian Staehelin4
1Microbiology Department, Soils, Water and Environment Research Institute, ARC, Egypt.
2Botany Department, Faculty of Science, Tanta Univ. Egypt.
3Biotechnology Institute, College of Biological Sciences, University of Minnesota, Saint Paul, USA
4School of Life Sciences, State Key Laboratory of Biocontrol, Sun Yat-sen (Zhongshan) University, East Campus, Guangzhou 510006, China.
Abstract
Eighteen isolates of Azospirillum were collected from different sites at Kafrelsheikh Governorate. The isolates were cultivated on nitrogen-free malate (NFM) medium supplemented with different NaCl gl-1 concentrations (Zero, 0.7 and 1.5%) and tryptophan to study the salt tolerant in vitro and indol acetic acid production. Pots were used to evaluate the most salt-tolerant and efficient species and soils of this experiment had artificially salinized with different salinity levels. The most salt-tolerant A10 and A11 species were genetically identified by 16S rRNA sequencing and the results revealed to be closest matched at 99% A. lipoferum strains and selected to inoculate wheat plants. The investigated A. lipoferum species had variable divergence growth at different salinity levels. Actually, increased salinity had deleterious effect on IAA production. In addition, salinity had deleterious effect on dry weights of plants, number and dry weight of branches, total chlorophyll, sodium and potassium% with increasing salinity levels. But, inoculation with associative nitrogen fixation A. lipoferum isolates enhanced the previous parameters. Thus, inoculation with the salt-tolerant A.lipoferum isolates decreased the deleterious effect of salt stress on wheat plants.
Keywords
salinity; Wheat; 16srRNA; Azospirillum lipoferum; IAA
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