Accumulative persistence of the genotoxic and mutagenic effects induced by low doses of TiO2 nanoparticles increases the incidence of hepatocellular carcinoma in mice
Hamad Mohamed, H., Tharwat, A., Edwar, S., Samir, L., Salag, E., Tarek, O. (2019). Accumulative persistence of the genotoxic and mutagenic effects induced by low doses of TiO2 nanoparticles increases the incidence of hepatocellular carcinoma in mice. EKB Journal Management System, 1(1), 29-47. doi: 10.21608/rrgg.2019.56175
Hanan Ramadan Hamad Mohamed; Andrew Tharwat; Sara Edwar; Lidia Samir; Engy Salag; Omar Tarek. "Accumulative persistence of the genotoxic and mutagenic effects induced by low doses of TiO2 nanoparticles increases the incidence of hepatocellular carcinoma in mice". EKB Journal Management System, 1, 1, 2019, 29-47. doi: 10.21608/rrgg.2019.56175
Hamad Mohamed, H., Tharwat, A., Edwar, S., Samir, L., Salag, E., Tarek, O. (2019). 'Accumulative persistence of the genotoxic and mutagenic effects induced by low doses of TiO2 nanoparticles increases the incidence of hepatocellular carcinoma in mice', EKB Journal Management System, 1(1), pp. 29-47. doi: 10.21608/rrgg.2019.56175
Hamad Mohamed, H., Tharwat, A., Edwar, S., Samir, L., Salag, E., Tarek, O. Accumulative persistence of the genotoxic and mutagenic effects induced by low doses of TiO2 nanoparticles increases the incidence of hepatocellular carcinoma in mice. EKB Journal Management System, 2019; 1(1): 29-47. doi: 10.21608/rrgg.2019.56175

Accumulative persistence of the genotoxic and mutagenic effects induced by low doses of TiO2 nanoparticles increases the incidence of hepatocellular carcinoma in mice

Recent Research in Genetics and Genomics
Article 3, Volume 1, Issue 1, 2019, Page 29-47  XML PDF (1.27 MB)
Document Type: Original Research Articles
DOI: 10.21608/rrgg.2019.56175
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Authors
Hanan Ramadan Hamad Mohamed email orcid 1; Andrew Tharwat2; Sara Edwar3; Lidia Samir4; Engy Salag5; Omar Tarek6
1Zoology department faculty of science Cairo university Giza Egypt
2Biotechnology/Bimolecular student Faculty of Science Cairo university
3Biotechnology/Bimolecular student Faculty of Science Cairo University
4Biotechnology/Bimolecular student Faculty of science Cairo university
5Biotechnology/ Bimolecular student Faculty of science Cairo university
6Biotechnology/Bimolecular student Faculty of science Cairo
Abstract
Nowadays, titanium dioxide nanoparticles (TiO2NPs) are widely used by many industries and are present in many products including toothpastes, sweets, and medications. Therefore, humans are exposed to considerable amounts of this material in their daily lives. However, limited studies are available regarding the relationship between the effects of TiO2NP-induced genotoxicity and the incidence of liver cancer. Therefore, this study was designed to investigate the impact of TiO2NPs-induced genotoxicity and mutagenicity in the livers of mice. Further, we assessed the development of hepatic cancer in these mice. Oral administration of TiO2NPs resulted in persistent single and double stranded DNA breaks which were indicated by the fragmented and smeared genomic DNA observed on an agarose gel. This damaged DNA resulted in apoptosis-induced liver damage. Progressive accumulation of TiO2NPs-induced DNA damage resulted in a high mutation incidence in both the p53 and H-ras genes. Moreover, histological examinations revealed necrosis throughout the liver during the experimental period. This damage could be attributed to the persistent and significant increase in malondialdehyde and reduction in glutathione levels. In conclusion, the accumulation of orally administered TiO2NPs resulted in persistent DNA damage, apoptosis, and oxidative stress. These events ultimately lead to necrosis in the entire liver, which increased the incidence of hepatocellular carcinoma.
Keywords
DNA damage; bio-persistence of TiO2nanoparticles; p53; H ras; Hepatocellular carcinoma; oxidative stress and mice
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