The Possible Therapeutic Effect of Mesenchymal Stem Cells and their Exosomes on Experimentally Induced Diabetic Retinopathy in Rats: Histological and Immunohistochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Article 3, Volume 43, Issue 2, June 2020, Page 390-411 PDF (20.13 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2019.18175.1185 | ||||
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| Authors | ||||
heba Elsayed Abd El-Halim  1; omyma kamel helal2; nesrien ebrahim salem3; nahla eleraky elazab4
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| 1benha faculty of medicine DEPARTMENT OF HISTOLOGY | ||||
| 2Histology and Cell Biology Department, Faculty of Medicine , Benha Univeristy, Benha, Egypt | ||||
| 3Histology and Cell Biology , Benha faculty of Medicine , Benha University, Egypt | ||||
| 4Histology and Cell Biology Department, Faculty of Medicine , Benha University, Benha, Egypt | ||||
| Abstract | ||||
| Background: Diabetic retinopathy (DR) is one of the most common microvascular complication of diabetes that affects the retina and causes acquired blindness among working-age people. Stem cell therapy and exosomes have become promising therapeutic strategies for DR with the development of modern medical technology in the field of cell therapy. Objective: To evaluate the possible potential therapeutic effect of bone marrow derived Mesenchymal stem cells (BMMSCs) and their exosomes (BMMSCs-exosomes) on induced diabetic retinopathy in rats. Materials and Methods: Ten young rats were used to prepare mesenchymal stem cells (MSCs). Sixty-four adult male albino rats were divided into six groups. Group I (Control group). Group II (affected group): Rats received single intraperitoneal injection of STZ (60 mg/kg body weight), freshly dissolved in citrate buffer. Group III: DR treated with BMMSCs. Group IV: DR treated with BMMSCs-exosomes. Group V: DR treated with BMMSCs and MSCs-exosomes. Group VI (recovery group). Retinal specimens were taken and processed for histological and immunohistochemical examination. Results: Group II and VI displayed decreased retinal thickness, obvious disorganization of the outer segment of photoreceptors, together with cytoplasmic vacuolations in the cells of the inner nuclear and ganglionic layers. Furthermore, there was a significant increase (P < 0.05) in VEGF and vimentin immunoexpression. Groups III and IV showed improvement of some histological microscopic changes described in group II. While, group V displayed histological architecture and ultrastructure near to control group. Conclusion: MSCs and exosomes can treat diabetic retinopathy. However, better results can be obtained when exosomes were given with MSCs. | ||||
| Keywords | ||||
| Diabetic retinopathy; MSCs; exosomes; VEGF and vimentin | ||||
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