GENOTYPIC EFFECTS, OXIDATIVE STRESS AND TOLERANCE MECHANISMS INDUCED BY CADMIUM IN TWO Lactuca sativa CULTIVARS.
El-Kafafi, E., El-Demerdash, F., Helaly, A., El-Sheikha, M. (2011). GENOTYPIC EFFECTS, OXIDATIVE STRESS AND TOLERANCE MECHANISMS INDUCED BY CADMIUM IN TWO Lactuca sativa CULTIVARS.. EKB Journal Management System, 2(7), 139-153. doi: 10.21608/jacb.2011.57083
E. El-Kafafi; Fatma M. El-Demerdash; A. A. Helaly; M. El-Sheikha. "GENOTYPIC EFFECTS, OXIDATIVE STRESS AND TOLERANCE MECHANISMS INDUCED BY CADMIUM IN TWO Lactuca sativa CULTIVARS.". EKB Journal Management System, 2, 7, 2011, 139-153. doi: 10.21608/jacb.2011.57083
El-Kafafi, E., El-Demerdash, F., Helaly, A., El-Sheikha, M. (2011). 'GENOTYPIC EFFECTS, OXIDATIVE STRESS AND TOLERANCE MECHANISMS INDUCED BY CADMIUM IN TWO Lactuca sativa CULTIVARS.', EKB Journal Management System, 2(7), pp. 139-153. doi: 10.21608/jacb.2011.57083
El-Kafafi, E., El-Demerdash, F., Helaly, A., El-Sheikha, M. GENOTYPIC EFFECTS, OXIDATIVE STRESS AND TOLERANCE MECHANISMS INDUCED BY CADMIUM IN TWO Lactuca sativa CULTIVARS.. EKB Journal Management System, 2011; 2(7): 139-153. doi: 10.21608/jacb.2011.57083

GENOTYPIC EFFECTS, OXIDATIVE STRESS AND TOLERANCE MECHANISMS INDUCED BY CADMIUM IN TWO Lactuca sativa CULTIVARS.

Journal of Agricultural Chemistry and Biotechnology
Article 2, Volume 2, Issue 7, July 2011, Page 139-153  XML PDF (447.64 K)
Document Type: Original Article
DOI: 10.21608/jacb.2011.57083
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Authors
E. El-Kafafi1; Fatma M. El-Demerdash2; A. A. Helaly3; M. El-Sheikha1
1Botany Dept., Fac. Agric., Al-Azhar University, Nasr City, Cairo, Egypt.,
2Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt
3Horticulture Dept., Fac. Agric., Al-Azhar Univ., Nasr City, Cairo, Egypt.
Abstract
A hydroponic experiment was carried out to investigate the effect of cadmium (Cd) on differential expression, oxidative stress and some antioxidant enzyme activities in two Lactuca sativa cultivars (Balady, related Romaine Group and Great leaks, related Crisp Group). Cd phytotoxicity was shown by growth retardation of Balady and Great leaks. Results showed that Great leaks showed more sensitivity to Cd toxicity than Balady cultivar. Increasing Cd supply markedly reduced the total chlorophyll, dry matter of both Lactuca cultivarsand these decreases were more marked in great leaks. Increased Cd accumulation in various plant parts in both the Lactuca cultivars was observed as Cd concentration increased. Cd accumulated in the roots was much higher than in the shoot in the two cultivars, but more observed in the case of Balady cultivar. Balady cultivar had less uptake of Cd by shoot about two times than Great leaks shoot cultivar indicating that there are co-ordination of gene expression, regulation ion transport process operating in different root layer more efficient in Balady cultivar compared with Great leaks. Moreover, the induction of expression and activities of antioxidant enzymes and non protein thiol (NPT) increased in Balady cultivar more than Great leaks leading to H2O2 burst, lipid peroxidation, and growth inhibition. These gene expression and activities of antioxidant enzymes confer Balady cultivar some measure of Cd tolerance and presence of strong Cd-binding proteins in the roots. In conclusion, these results may be regarded as an indication of better tolerance mechanism of Balady cultivarmore than that of Great leaks to Cd contamination.
Keywords
Lactuca sativa; Cadmium toxicity; Oxidative Stress; biomarkers; Phytotoxicity
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