A Histological Study of the Effect of Aspartame Versus Sucralose on the Spleen of Adult Male Albino Rats | ||||
| The Egyptian Journal of Anatomy | ||||
| Article 7, Volume 40, Issue 1, January 2017, Page 107-119 PDF (10.66 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejana.2017.5716 | ||||
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| Author | ||||
| Shereen Saad | ||||
| Anatomy department, Faculty of Medicine, Ain Shams University | ||||
| Abstract | ||||
| Introduction: Aspartame (ASP) is one of the most widely artificial sweeteners consumed worldwide. Some studies declared that administration of ASP even at the Food and Drug Administration permitted level causes oxidative stress by altering the oxidant /antioxidant balance in immune organs of the rats. On the other hand, sucralose is another artificial high-potency sweetening compound. It has been branded under the name Splenda. It is found in many food and beverage products. Studies observed that sucralose administration caused reduction of weight of the spleen and histopathological changes in the thymus of rats. Aim of the work was to study the histological and ultrastructural changes of the spleen of adult male albino rats, upon exposure to ASP and sucralose. Materials and Methods: thirty male albino rats were divided into three groups Group I control group: ten adult rats received 1 ml distilled water daily for 3 months. Group II (Aspartame group): ten adult rats administered aspartame (40 mg/kg) daily dissolved in 1 ml distilled water daily for 3 months. Group III (sucralose group): ten rats received sucralose 15 mg/kg daily dissolved in 1 ml distilled water daily for 3 months. At the end of the experiment, spleen specimens were dissected and processed for Both light microscope and ultra-structural examination. Results: Histological examination of Hx. & E sections of the spleen of ASP group revealed loss of architecture of the spleen accompanied by partial disappearance of demarcation between white and red pulps. Lymphoid follicles showed depletion of lymphocytes. Examination of the ultrathin sections showed degenerated lymphocytes in the white pulp, with highly condensed pyknotic nuclei. In addition, cytoplasm revealed the presence of vacuoles. On the other hand, examination of Hx. & E of the spleen of sucralose group revealed complete loss of architecture of spleen with disappearance of demarcation between red and white pulps. Marked areas of depletion of lymphocytes in both red and white pulps and elongated and thickened fibrous tissue strands were observed. Examination of the ultrathin sections showed extensive red pulp with degenerated white pulp. Degenerated lymphocytes with irregular indented nuclei with widening of peri-nuclear space. In addition degenerated cytoplasm, many vacuoles and esinophil infiltration. Conclusion: The present study clearly points that both ASP and sucralose can influence the structure of the spleen within the acceptable daily consumption. Whereas sucralose had more degenerative effects on the spleen. | ||||
| Keywords | ||||
| Aspartame; sucralose; spleen | ||||
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