Protective effects of black seed and vitamin C on tamoxifen induced liver changes in adult female albino rat; biochemical and microscopic study | ||||
| The Egyptian Journal of Anatomy | ||||
| Article 11, Volume 40, Issue 1, January 2017, Page 155-171 PDF (15.33 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejana.2017.5720 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Rania Sherif; Huda Eltahry; Sara Abu bakr | ||||
| Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt | ||||
| Abstract | ||||
| INTRODUCTION: Tamoxifen is one of the ideal drugs in treatment of breast cancer however it has genotoxic and cytotoxic activity on the liver via producing reactive oxygen species. AIM: To investigate the injurious effect of tamoxifen on liver of the adult female albino rats and to find out the possible protective effect of Black Seed and/or Vitamin C against this hepatic injury. METHODS: Thirty adult female albino rats were randomly divided into 5 groups;control, tamoxifen, tamoxifen + blackseed, tamoxifen +vitamin C and tamoxifen +blackseed and vitamin C treated groups. On the 22nd day after tamoxifen administrationthe rats were sacrificed; the livers wereprocessed for measurement of MDA &SOD and for light and electron microscopic examination. RESULTS: Co-administration of black seed and/or vitamin C with tamoxifen caused significant decrease in the elevated serum GPT and GOT and the liver tissue MDA and increase in the decreased liver tissue SOD when compared with tamoxifen group. By Light microscopic examination, tamoxifen has been found to cause several liver changes in the form of hepatocyte degeneration and necrosis, inflammatory cell infiltration, dilated sinusoids with hypertrophy of Kupffer cells, collagen deposition and nuclear changes as chromatin clumping and trizomy. Electron microscopic examination of tamoxifen group revealed nuclear changes, dilated vesicular rER, secondary lysosomes, swollen mitochondria and lipid droplets. Administration of black seed and/or vitamin C with tamoxifen reduced the hepatic pathological changes. CONCLUSION: Combination of both black seed and vitamin C might have synergistic hepato-protective effect. | ||||
| Keywords | ||||
| tamoxifen; blackseed; vitamin c; hepatocyte | ||||
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