Electrophoretic Patterns of Some Populations of Meloidogyne Arenaria and M. javanica | ||||
Egyptian Journal of Agronematology | ||||
Article 4, Volume 15, Issue 1, January 2016, Page 44-52 PDF (302.26 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejaj.2016.57479 | ||||
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Authors | ||||
Mahfouz Abd-Elgawad1; Mohamed Eissa1; Abd-Elmoneim El-Gindi2; Ahmed Osman2; Grover Smart3; Ahmed El-bahrawy4 | ||||
1Phytopathology Department, National Research Centre; | ||||
2Department of Agricultural Zoology and Nematology, Faculty of Agriculture, University of Cairo, Giza, Egypt | ||||
3Department of Entomology and Nematology, IFAS, University of Florida, USA | ||||
4Institute for Sustainable Plant Protection, National Council of Research, Bari, Italy. | ||||
Abstract | ||||
Root-knot nematodes (Meloidogyne spp.) are important polyphagous plant parasites worldwide. Intraspecific variation and the occurrence of physiological races have necessitated the development of taxonomic techniques enabling differentiation and classification of races related to major Meloidogyne spp. Accurate identification of their races and populations is essential for determining appropriate management approaches and benefits from further characterization and understanding of the variability within a single nematode species. Electrophoretic focusing of malic dehydrogenase, α-glycerophosphate dehydrogenase and esterase for seven populations of Meloidogyne spp. by polyacrylamide gels was tested. These populations included 1, 2 and 4 populations of M. javanica and M. arenaria race I and II, respectively. Malic Dehydrogenase profiles obtained by polyacrylamide-gel electrophoresis indicated that differences between Meloidogyne arenaria and M. javanica were more pronounced than were differences between races or populations of M. arenaria. The enzyme patterns of the two species varied even though the nematodes were propagated on the same host plant (Rutgers tomato). | ||||
Keywords | ||||
Biochemical markers; Meloidogyne arenaria; M. javanica; root-knot nematode; molecular taxonomy | ||||
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