Quercetin Supplementation Ameliorates Myopathy Associated with Induced Type 2 Diabetes Mellitus in Rats | ||||
| The Medical Journal of Cairo University | ||||
| Article 28, Volume 86, September, September 2018, Page 2341-2347 PDF (527.83 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2018.57537 | ||||
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| Authors | ||||
| SOHEIR A. SALEH, M.D.; MOHAMED HANAFY, M.D.; SUZY F. EWIDA, M.D.; MOHAMED A. ZAYED, M.D.; SUZAN A.A. KHODIR, M.Sc. | ||||
| The Department of Physiology, Faculty of Medicine, Menoufia University, Egypt* and Rabigh Branch, King Abdul Aziz University, Saudi Arabia** | ||||
| Abstract | ||||
| Abstract Background: Myopathy is a common associated diabetic complication due to hyperglycemia and insulin resistance. Quercetin acts as reactive oxygen species' scavenger and peroxidation reactions' suppressor which causes development of diabetic complications. Aim of Study: We investigated the possible improving effect quercetin on glycemic state, insulin resistance & skeletal muscle performance in type 2 diabetic male rats and tried to elucidate the possible underlying mechanisms. Patients and Methods: Thirty rats were randomly divided into three groups; non-diabetic, diabetic non-treated & quer-cetin treated groups. Type 2 diabetes mellitus was induced by adding 10% fructose for 2 weeks followed by single intraperitoneal injection of streptozotocin as a consequence of hyperglycemic insulin resistance. Quercetin was given in a dose of 50mg/kg body weight orally for 4 weeks. After the end of the 4-week experimental period, fasting serum glucose, glycosylated hemoglobin, fasting serum insulin, HOMA-IR index, serum TNF-a, MDA, total antioxidant capacity, Gast wt/Tib L ratio, the strength of muscle contraction at the start and after 30min activity & glucose uptake by the muscle during 30min activity and after 30min recovery were assessed. Results: Fructose & Streptozotocin administration signif-icantly increased fasting serum glucose, glycosylated hemo-globin (HbA1C), fasting serum insulin, HOMA IR index, confirming successful establishment of Type 2 diabetes Mel-litus model. Also increased serum MDA, TNF-a and decreased total antioxidant capacity associated with decreased Gast wt/ Tib L ratio, decreased the strength of muscle contraction at the start and after 30min activity and reduced glucose uptake by the muscle during 30min activity and after 3 min recovery indicating occurrence of myopathy. Quercetin supplementation significantly improved the previously measured parameters compared to diabetic non-treated group. Conclusion: Quercetin improving effect against myopathy complicating induced type 2 diabetes mellitus may be linked to its antioxidant and anti-inflammatory effect with reduced hyperglycemia and insulin resistance. | ||||
| Keywords | ||||
| Quercetin – Fructose – Streptozotocin – Type 2 diabetes – Anti-inflammatory – Antioxidant | ||||
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