EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) IN HEMANGIOMAS AND INTERMEDIATE VASCULAR LESIONS | ||||
| Alexandria Dental Journal | ||||
| Article 1, Volume 43, Issue 2, August 2018, Page 1-6 PDF (817.72 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjalexu.2018.57615 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ahmed E Zyton1; Hanaa S Raslan2; Omneya R Ramadan3; Azza Z Abdel Rahman4 | ||||
| 1Master student of Oral Pathology, Faculty of Dentistry-Alexandria University | ||||
| 2Professor of Oral Pathology Department, Faculty of Dentistry-Alexandria University | ||||
| 3Lecturer of Oral Pathology Department, Faculty of Dentistry-Alexandria University | ||||
| 4Professor of Oral Medicine Department, Faculty of Dentistry-Alexandria University | ||||
| Abstract | ||||
| between hemangiomas and angiosarcomas. Angiogenesis is characterized by development of new blood vessels through the division or migration of pre-existing vasculature. VEGF deserves attention because it is associated with the stimulation of endothelial cell proliferation and vascular permeability and promotion of angiogenesis. OBJECTIVES: to evaluate the immunohistochemical expression of vascular endothelial growth factor (VEGF) in pyogenic granulomas, hemangiomas and intermediate vascular tumors (hemangioendotheliomas, hemangiopericytomas) and to highlight the possible role of VEGF in diagnosis of each of these tumors MATERIALS AND METHODS: Immunohistochemical analysis of 20 cases of vascular and intermediate vascular tumors, with one section from each specimen (20 sections for VEGF antibody) and 5 control cases. Immunohistochemical staining was performed using a Labeled Strept-Avidin Biotin method (LSAB). RESULTS: Normal gingival tissue showed mild immunoreactivity for VEGF. All the examined cases showed strong positive expression for VEGF antibody, with different intensities. CONCLUSIONS: The marker VEGF was overexpressed in vascular and intermediate vascular tumors than normal gingival tissue | ||||
| Keywords | ||||
| Vascular tumors; Intermediate vascular tumors; Immunohistochemistry; VEGF | ||||
| References | ||||
|
1. Richter GT, Friedman AB. Hemangiomas and vascular malformations: current theory and management. Int J Pediatr 2012; 2012: 645-78.
2. Sedivy R, Beck-Managetta J, Haverkam PF. Expression of vascular endothelial growth factor-C correlates with the lymphatic microvessel density and the nodal status in oral squamous cell cancer. J Oral Pathol Med 2003; 32: 455-60.
3. Carmeliet P, Jain RK. Angiogenesis in cancer and other diseases. Nature 2000; 407: 249-57.
4. Vermeulen PB, Gasparini G, Foks SB, Colpaert C, Marson LP, Gion M, et al. Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. Eur J Cancer 2002; 38: 1564-79
5. Cohen MM Jr. Vasculogenesis, angiogenesis, hemangiomas, and vascular malformations. Am J Med Genet 2002; 108: 265-74.
6. Pajusola K, Aprelikova O, Korhonen J, Kaipainen A, Pertovaara L, Alitalo R, et al. FLT4 receptor tyrosine kinase contains seven immunoglobulin-like loops and is expressed in multiple human tissues and cell lines. Cancer Res 1992; 52: 5738-43
7. Zhou L, Xu J, Kang J. matrix metalloproteinase-9 and vascular endothelial growth factor. Expression of matrix metalloproteinase-2, in retinoblastoma and its clinical significance. Yan Ke Xue Bao 2010; 25: 62-4.
8. Stacher E, Gruber-Mosenbacher U, Halbwedl I, Dei Tos AP, Cavazza A, Papotti M. The VEGF-system in primary pulmonary angiosarcomas and haemangioendotheliomas: new potential therapeutic targets? Lung Cancer 2009; 65: 49-55.
9. Zheng S, Han MY, Xiao ZX, Peng JP, Dong Q. Clinical significance of vascular endothelial growth factor expression and neovascularization in colorectal carcinoma. World J Gastroenterol 2003; 9: 1227-30.
10. Bancroft JD, Stevens A. theory and practice of histological techniques. 3th ed. Churchill livingstone inc. 1990.
11. Grizzle WE. Models of fiation and tissue processing. Biotech Histochem 2009; 84: 185-93
12. Brouillard P, Vikkula M. Vascular malformations: localized defects in vascular morphogenesis. Clin Genet 2003; 63: 340.
13. Saghafi S, Amoueian S, Montazer M, Bostan R. Assessment of VEGF, CD-31 and Ki-67 in Oral Pyogenic Granuloma: A Comparison with Hemangioma and Inflammatory Gingivitis. Iranian J Basic Med Sci 2011; 14: 185-9.
14. Jafarzaddeh H, Sanatkhani M, Mohtasham N. Oral pyogenic granuloma: a review. J Oral Sci 2006; 48: 167-75.
15. Toida M, Hasegawa T, Watanabe F, Kato K, Makita H, Fujitsuka H, et al. Lobular capillary hemangioma of the oral mucosa: clinicopathological study of 43 cases with a special reference to immunohistochemical characterization of the vascular elements. Pathol Int 2003; 53: 1-7.
16. Reis-Filho JS, Paiva ME, Lopes JM. Congenital composite hemangioendothelioma: case report and reappraisal of the hemangioendothelioma spectrum. J Cutan Pathol 2002; 29: 226-31.
17. Bhutia O, Roychoudhury A. Hemangiopericytoma of mandible. J Oral Maxillofac Pathol 2008; 12: 26-8.
18. Rizwanulla T, Koirala B, Shivalal Sharma S, Adhikari L. Klebsiella ozaenae Cholecystitis. Am J Med Sci 2010; 8: 196-8.
19. Zha-jun FU, Chun-ming LI, Wang T, Jiang Z, Zhao-Chen FU. Vascular endothelial growth factor expression and pathological changes in the local tissue of facial hemangiomas following injections with pure alcohol. Oncol Lett 2015; 9: 1099-103.
20. Quante M, Patel NK, Hill S, Merchant W, Courtauld E, Newman P, et al. Epithelioid hemangioendothelioma presenting in the skin: a clinicopathologic study of eight cases. Am J Dermatopathol 1998; 20: 541-6.
21. Carvalho JR, Haddad L, Leonhardt FD, Filho MFM, Santos RO, Cervantes O, et al. Hemangiopericitoma maligno de cabeça e pescoço em uma criança: relato de caso. São Paulo Med J 2004; 122: 223-6.
22. Hasina R, Lingen MW. Angiogenesis and oral cancer. J Dent Educ 2001; 65: 1282-90. 23. Kumar V, Abbas AK, Fausto N. Robbins and Cotran Pathologic basis of disease. 7th ed. Philadelphia; Saunders: Elsevier Inc, 2004. RB111.R62.
24. Hand JL, Frieden IJ. Vascular birthmarks of infancy: Resolving nosologic confusion. Am J Med Genet 2002; 108: 257-264 .
25. Yuan K, Wing TJ, Lin MT. The detection and comparison of angiogenesis-associated factors in pyogenic granuloma by immunohistochemistry. J Periodontol 2000; 71: 701-9.
26. Ou JM, Yu ZY, Qiu MK, Dai YX, Dong Q, Shen J, et al. Knockdown of VEGFR2 inhibits proliferation and induces apoptosis in hemangioma derived endothelial cell. Eur J Histochem 2014; 58: 2263.
27. Miettinen M, Rikala M, Rysz J. VEGF receptor2. As marker for malignant vascular tumors and mesothelioma. Immunohistochemical study of 262 vascular endothelial and 1640 nonvascular tumors. Am J Surg Pathol 2012: 35: 629-39.
28. Zhang L, Lin X, Wang W, Dong J, Qi Z, Hu Q. Circulating level of vascular endothelial growth factor in differentiating hemangioma from vascular malformation patients. Plast Reconstr Surg 2005; 116: 200-4.
29. Jinnin M, Medici D, Park L, Limaye N, Liu Y, Boscolo E, et al. Uppressed NFAT-dependent VEGFR1 expression and constitutive VEGFR2 signaling in infantile hemangioma. Nat Med 2008; 14: 1236-46.
30. Dietzmann K, von Bossanyi P, Warich-Kirches M, Kirches E. Immuno-histochemical detection of vascular growth factors in angiomatous and atypical meningiomas, as well as hemangiopericytomas. Pathol Res Pract 1997; 193: 503-10.
31. Gengler C, Guillou L. Solitary fibrous tumour and haemangiopericytoma: evolution of a concept. Histopathology 2006; 48: 63-74.
32. Hatva E, Bohling T, Jaaskelainen J, Paetau A, Haltia M, Volk B, et al. Vascular growth factors and receptors in capillary hemangioblastomas and hemangiopericytomas. Am J Pathol 1996; 148: 763-75.
33. Vermeulen PB, Gasparini G, Foks SB, Colpaert C, Marson LP, Gion M, et al. Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. Eur J Cancer 2002; 38: 1564-79.
34. Dyduch G, Okoń K, Mierzyński W. Benign vascular proliferations--an immune-histochemical and comparative study. Pol J Pathol 2004; 55: 59-64. 35. Hasan J, Byers R, Jayson GC. Intra-tumoural microvessel density in human solid tumors. Br J Cancer 2002; 86: 1566-77.
36. Pakos EE, Goussia AC, Tsekeris PG, Papachristou DJ, Stefanou D, Agnantis NJ. Expression of vascular endothelial growth factor and its receptor. KDR/Flk-1, in soft tissue sarcomas. Anticancer Res 2005; 25: 3591- 6.
37. Woessner RD, Wright PS, Loudy DE, Wallace CD, Montgomery LR, Nestok BR. Microautoradiographic quantitation of vascular endothelial growth factor mRNA levels in human prostate specimens containing normal and neoplastic epithelium. Exp Mol Pathol 1998; 65: 37-52.
38. Tae K, El-Naggar AK, Yoo E, Feng L, Lee JJ, Hong WK, et al. Expression of vascular endothelial growth factor and microvessel density in head and neck tumorigenesis. Clin Cancer Res 2000; 6: 2821-8. | ||||
|
Statistics Article View: 137 PDF Download: 414 |
||||
