Additive Effect of Chitinase Genes in Transconjugants of Entomopathogens Bacillus thuringiensis to Improve Biological Control of Tuta absoluta | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 3, Volume 10, Issue 8, August 2019, Page 159-170 PDF (830.21 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2019.57723 | ||||
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| Authors | ||||
| هبه حامد عطيه1; ميرفت إبراهیم کمال2; أحمد ابراهیم السيد1; على ماهر محمد العدل2; خليفه عبد المقصود زايد3 | ||||
| 1معهد بحوث الأراضى والمياه والبيئة – مرکز البحوث الزراعية بالجيزة | ||||
| 2قسم الوراثة – کلية الزراعة – جامعة المنصورة | ||||
| 31قسم الوراثة – کلية الزراعة – جامعة المنصورة | ||||
| Abstract | ||||
| This study aimed to evaluate the toxicity of Bacillus thuringiensis (Bt) transconjugants harboring chitinase genes against larval stage of Tuta absoluta . Bacillus licheniformisand Serratia marcescens were used as donors against Bt . Bacillus transconjugants were evaluated for chitinase activity depending on hydrolysis zone appeared on chitin agar medium. Parental strains and their recombinants significantly reduced the number of survived larvae if compared with untreated larvae. Mortality rate was gradually increased to reached a maximum ratio after six days of treatments. Most bioinsecticides including parental strains and their transconjugants significantly losses larval body weight daily if compared with untreated larvae.Bioinsecticides, as well as, bioinsecticide concentrations and the interaction between both factors showed negative significant effect on larval body weight at most time intervals of larval age.In addition, Bt recombinants , as well as, their parental strains has exhibited a great potential effect for controlling and suppress the feeding of larval stage on the host plants. | ||||
| Keywords | ||||
| Bacillus thuringiensis; conjugation; larval mortality; larval weight; Tuta absoluta | ||||
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