EFFICACY OF QUERCETIN ON ALVEOLAR BONE STRUCTURE OF RATS WITH INDUCED DIABETES | ||||
| Alexandria Dental Journal | ||||
| Article 3, Volume 42, Issue 2, December 2017, Page 141-146 PDF (1.41 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjalexu.2017.57917 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mariam A. Abu Ayana* 1; Nawal A. Elmasry2; Fathy I. Shehata2; Nesma M. Khalil3 | ||||
| 1Demonstrator of oral biology, Faculty of dentistry, Alexandria University | ||||
| 2Professor of oral biology, Faculty of Dentistry, Alexandria University. | ||||
| 3Lecturer of oral biology, Faculty of Dentistry, Alexandria University. | ||||
| Abstract | ||||
| INTRODUCTION: Diabetic osteopenia is the sixth classic complication of Diabetes mellitus. High glucose level is capable of triggering increased oxidative stress which induce osteoblast apoptosis. Quercetin, a powerful natural antioxidant, can protect many organs against oxidative damage, including bone. OBJECTIVES: The present study is designed to investigate the effect of quercetin administration on the alveolar bone in rats with induced diabetes. MATERIALS AND METHODS: Twenty-four adult male rats were divided into three equal groups (8 rats each) as follows: Group I: Control group, Group II: diabetic group (with no treatment), Group III: quercetin treated group (induction of diabetes with oral administration of quercetin dose 100mg/ kg/ day for 12 weeks). Diabetes was induced in group II and group III by a single intraperitoneal injection of Streptozotocin (STZ). After 12 weeks all rats were sacrificed and the mandibles were dissected out and prepared for histological analysis using scanning electron microscope (SEM), and energy dispersive x-ray microanalysis (EDX). RESULTS: In diabetic group, there was significant increase in blood sugar level. Bone surface revealed irregular surface with multiple resorptive pits. Moreover, there was significant decrease in calcium level and increase in phosphorous level while in quercetin group. Both blood sugar level and alveolar bone surface relatively returned back to normal. Also there was slight decrease in calcium and increase in phosphorous in comparison to control group. CONCLUSIONS: Quercetin exerts protective effects on STZ-induced oxidative stress in diabetic rats restoring the architecture of bone. | ||||
| Keywords | ||||
| Quercetin; streptozotocin; diabetes; alveolar bone | ||||
| References | ||||
|
1. Yokoyama A, Yokoyama A, Sakakibara H, Yokoyama A, Sakakibara H, Crozier A, et al. Quercetin metabolites and protection against peroxynitrite-induced oxidative hepatic injury in rats. Free Radic Res. 2009;43:913-21.
2. Seiva FR, Chuffa LGA, Braga CP, Amorim JPA, Fernandes AAH. Quercetin ameliorates glucose and lipid metabolism and improves antioxidant status in postnatally monosodium glutamate-induced metabolic alterations. Food Chem Toxicol. 2012;50:3556-61.
3. Lai F, Franceschini I, Corrias F, Sala MC, Cilurzo F, Sinico C, et al. Maltodextrin fast dissolving films for quercetin nanocrystal delivery. A feasibility study. Carbohydr Polym. 2015;121:217-23.
4. Welch A, MacGregor A, Jennings A, Fairweather-Tait S, Spector T, Cassidy A. Habitual flavonoid intakes are positively associated with bone mineral density in women. J Bone Miner Res. 2012;27:1872-8.
5. Huang Q, Gao B, Jie Q, Wei BY, Fan J, Zhang HY, et al. Ginsenoside-Rb2 displays anti-osteoporosis effects through reducing oxidative damage and bone-resorbing cytokines during osteogenesis. Bone. 2014;66:306-14.
6. Youl E, Bardy G, Magous R, Cros G, Sejalon F, Virsolvy A, et al. Quercetin potentiates insulin secretion and protects INS-1 pancreatic β-cells against oxidative damage via the ERK1/2 pathway. Br J Pharmacol. 2010;161:799-814
7. Khaki A, Fathiazad F, Nouri M, Khaki A, Maleki NA, Khamnei HJ, et al. Beneficial effects of quercetin on sperm parameters in streptozotocin-induced diabetic male rats. Phytother Res. 2010;24:1285-91.
8. Kobori M, Masumoto S, Akimoto Y, Takahashi Y. Dietary quercetin alleviates diabetic symptoms and reduces streptozotocin-induced disturbance of hepatic gene expression in mice. Mol Nutr Food Res. 2009;53:859-68.
9. Derakhshanian H, Ghadbeigi S, Rezaian M, Bahremand A, Javanbakht MH, Golpaie A, et al. Quercetin improves bone strength in experimental biliary cirrhosis. Hepatol Res. 2013;43:394-400.
10. Albert DA, Ward A, Allweiss P, Graves DT, Knowler WC, Kunzel C, et al. Diabetes and oral disease: implications for health professionals. Ann N Y Acad Sci. 2012;1255:1-15.
11. Fagot-Campagna A, Bourdel-Marchasson I, Simon D. Burden of diabetes in an aging population: prevalence, incidence, mortality, characteristics and quality of care. Diabetes Metab. 2005;31:5S35-52.
12. Gupta S, Mukherjee M. Diabetes mellitus and its treatment with some traditional herbs from the different districts of West Bengal: A Review. Int J PharmTech Res. 2014;6:1941-9.
13. Unnanuntana A, Rebolledo BJ, Khair MM, DiCarlo EF, Lane JM. Diseases affecting bone quality: beyond osteoporosis. Clin Orthop Relat Res. 2011;469:2194-206.
14. Koh WP, Wang R, Ang LW, Heng D, Yuan JM, Mimi CY. Diabetes and risk of hip fracture in the Singapore Chinese Health Study. Diabetes Care. 2010;33:1766-70.
15. Sodek J, Mckee MD. Molecular and cellular biology of alveolar bone. Periodontol 2000. 2000;24:99-126.
16. Zhen D, Chen Y, Tang X. Metformin reverses the deleterious effects of high glucose on osteoblast function. J Diabetes Complications. 2010;24:334-44.
17. Silva J, Lorencini M, Peroni L, De La Hoz C, Carvalho H, Stach-Machado D. The influence of type I diabetes mellitus on the expression and activity of gelatinases (matrix metalloproteinases-2 and -9) in induced periodontal disease. J Periodontal Res. 2008;43: 48-54.
18. Hamada Y, Fujii H, Fukagawa M. Role of oxidative stress in diabetic bone disorder. Bone. 2009;45:S35-8.
19. Mahmoud MF, Hassan NA, El Bassossy HM, Fahmy A. Quercetin protects against Diabetes-Induced Exaggerated Vasoconstriction in Rats: Effect on Low Grade Inflammation. PLoS One. 2013;8:e63784.
20. Ibuki FK, Simões A , Nogueira FN. Antioxidant enzymatic defense in salivary glands of streptozotocin-induced diabetic rats: a temporal study. Cell Biochem Funct. 2010;28:503-8.
21. Goldstein J, Newdury D, Joy D, Lyman C, Echlin P. Scanning electron microscope and X-ray analysis. 3rd ed. New York: Kluwer Academic/ Plenum publishers; 2003.
22. Zhou Y, Wu Y, Jiang X, Zhang X, Xia L, Lin K, et al. The effect of quercetin on the osteogenesic differentiation and angiogenic factor expression of bone marrow-derived mesenchymal stem cells. PloS One. 2015;10:e0129605.
23. Liang W, Luo Z, Ge S, Li M, Du J, Yang M, et al. Oral administration of quercetin inhibits bone loss in rat model of diabetic osteopenia. Eur J Pharmacol. 2011;670:317-24.
24. Dave V, Sharma R, Sharma S, Jain P, Yadav S. Experimental models on diabetes: a comprehensive review. IJAPR. 2013;4:1-8.
25. Hayashi K, Kojima R, Ito M. Strain differences in the diabetogenic activity of streptozotocin in mice. Biol Pharm Bull. 2006;29:1110-9.
26. Akbarzadeh A, Norouzian D, Mehrabi M, Jamshidi S, Farhangi A, Verdi AA, et al. Induction of diabetes by streptozotocin in rats. Indian J Clin Biochem. 2007;22:60- 4.
27. Motyl K, McCabe LR. Streptozotocin, type I diabetes severity and bone. Biol Proced Online. 2009;11:296-315.
28. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50:537-46.
29.Bortolin RH, Abreu BJdGA, Ururahy MAG, de Souza KSC, Bezerra JF, Loureiro MB, et al. Protection against T1DMinduced bone loss by zinc supplementation: biomechanical, histomorphometric, and molecular analyses in STZ-induced diabetic rats. PloS One. 2015;10:e0125349.
30.Coskun O, Kanter M, Korkmaz A, Oter S. Quercetin, a flavonoid antioxidant, prevents and protects streptozotocininduced oxidative stress and β-cell damage in rat pancreas. Pharmacol Res. 2005;51:117-23.
31. El-Amin M, Virk P, Elobeid M, Almarhoon ZM, Hassan ZK, Omer SA, et al. Anti-diabetic effect of Murraya koenigii (L) and Olea europaea (L) leaf extracts on streptozotocin induced diabetic rats. Pak J Pharm Sci. 2013;26:359-65.
32. Abdelmoaty MA, Ibrahim M, Ahmed N, Abdelaziz M. Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats. Indian J Clin Biochem. 2010;25:188-92.
33. Wongdee K, Charoenphandhu N. Osteoporosis in diabetes mellitus: Possible cellular and molecular mechanisms. World J Diabetes. 2011;2:41-8.
34. Labanca M, Binello PB. Osteoporosis and Bone Defects in Dentistry: New Drugs and Treatment Options. Open Conf Proc J. 2010;1:33-8.
35. Nagareddy PR, Lakshmana M. Assessment of experimental osteoporosis using CT-scanning, quantitative X-ray analysis and impact test in calcium deficient ovariectomized rats. J Pharmacol Toxicol Methods. 2005;52:350-5.
36.Berezin AE. Single Sample and Serial Measurements of Osteoprotegerin Level as a Target of Therapy in Type 2 Diabetes Mellitus? BMGT. 2016;3:57-71.
37. Shapiro R, Heaney R. Co-dependence of calcium and phosphorus for growth and bone development under conditions of varying deficiency. Bone. 2003;32:532-40.
38. Wong RW, Rabie ABM. Effect of quercetin on bone formation. J Orthop Res. 2008;26:1061-6.
39. Gupta SK, Kumar R, Mishra NC. Influence of quercetin and nanohydroxyapatite modifications of decellularized goatlung scaffold for bone regeneration. Mater Sci Eng C Mater Biol Appl. 2017;71:919-28.
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