LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)
Abd El-Wahed, A., Hassan, M., El-Hossary, W., Korraah, A. (2018). LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY). EKB Journal Management System, 43(3), 81-87. doi: 10.21608/adjalexu.2018.58003
Asmaa M Abd El-Wahed; Magda M Hassan; Wafaa H El-Hossary; Ahmed M Korraah. "LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)". EKB Journal Management System, 43, 3, 2018, 81-87. doi: 10.21608/adjalexu.2018.58003
Abd El-Wahed, A., Hassan, M., El-Hossary, W., Korraah, A. (2018). 'LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)', EKB Journal Management System, 43(3), pp. 81-87. doi: 10.21608/adjalexu.2018.58003
Abd El-Wahed, A., Hassan, M., El-Hossary, W., Korraah, A. LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY). EKB Journal Management System, 2018; 43(3): 81-87. doi: 10.21608/adjalexu.2018.58003

LOCALIZATION OF CYCLOOXYGENASE-2 IN EXPERIMENTALLY–INDUCED CARCINOGENESIS FOLLOWING TREATMENT WITH THYMOQUINONE LOADED ON NANO-GOLD PARTICLES. (A RETROSPECTIVE STUDY)

Alexandria Dental Journal
Article 14, Volume 43, Issue 3, December 2018, Page 81-87  XML PDF (860.38 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2018.58003
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Authors
Asmaa M Abd El-Wahed1; Magda M Hassan2; Wafaa H El-Hossary3; Ahmed M Korraah4
1Demonstrator in Oral Pathology department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
2Acting Head of Oral Pathology Department, Professor of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
3- Lecturer of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
4Lecturer of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
Abstract
Introduction: Thymoquinone is one of the phytochemicals used as chemopreventive agents for oral cancer control. TQ loaded on nanoparticles had shown higher anti-proliferative and anti-inflammatory effects than free TQ. Natural products that can inhibit Cyclooxygenase-2 (COX-2) would be a better choice in the prevention of tumor development with fewer side effects than pharmaceutical agents. Objective: To study the expression of Cox-2 in HBP/DMBA carcinogenesis model following combined topical application of TQ loaded on gold nanoparticles on alternative days. Material and Methods: This research was carried out on archival paraffin blocks of a previous thesis. The paraffin blocks represented hamster buccal pouches (HBPs) from 85 male Syrian golden hamsters were divided into 2 groups. The control groups included: I: negative control group, scarified at day zero then after 7 and 14 weeks, and II: positive control, treated with 7, 12-Dimethylbenz [a]-anthracene (DMBA) for 7 and 14 weeks. The experimental groups included: (III, IV and V) which were treated with the chemopreventive agents for 2 weeks then combined DMBA and the chemopreventive agents on alternative days for 7 and 14 weeks. Sections of 5µm were cut and processed for H&E and IHC stains for light microscopic study. Results: Negative and self-control groups showed negative COX-2 immunoreactivity. Group II showed intense Cox-2 immunoreactivity at both 7 and 14 weeks. Groups; III and IV showed moderate and intense Cox-2 immunoreactivity at 7 and 14 weeks, respectively. Group V showed mild and moderate Cox-2 staining reaction at 7 and 14 weeks, respectively. Conclusion: Topical application of G-NPs-TQ (0.001) for 7 and 14 weeks was able to reduce Cox-2 expression and in turn retard the carcinogenesis process due to its anti-inflammatory effect.
Keywords
Thymoquinone; Gold-nanoparticles; Oral squamous cell carcinoma; Cyclooxygenase-2; and Dimethylbenz-(a) anthracene
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