Targeting Cancer Immunotherapy through Vaccination Using Irradiated Xenogeneic Placental Extract | ||||
| Egyptian Journal of Radiation Sciences and Applications | ||||
| Article 17, Volume 31, Issue 1, July 2018, Page 77-87 PDF (774.35 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejrsa.2018.2694.1039 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Soheir A. A. Osman1; Nadia M. Abdallah2; Fatma F. Abdel Hamid3; Mohamed H. Nasr el-Dien4; Asmaa Abu Bakr5; Dalia A Mostafa  6
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| 1Prof. of biochemistry-NCRRT Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT)-Atomic Energy Authority (AEA), Cairo. | ||||
| 2Biochemistry Department, Faculty of Science, Ain Shams University, Egypt. | ||||
| 3Prof. of biochemestry-Ain Shams university Biochemistry Department, Faculty of Science, Ain Shams University, Egypt | ||||
| 4Faculty of Medicine, Ain Shams University. Egypt | ||||
| 5Dr. of biochemistry-NCRRT Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT)-Atomic Energy Authority (AEA), Cairo. | ||||
| 6Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT)-Atomic Energy Authority (AEA), Cairo. | ||||
| Abstract | ||||
| THIS STUDY investigates the injection of sterilized xenogeneic placental protein extract before challenge with viable Ehrlich tumor cells for enhancement of the immune system. It includes the estimation of tumor size, hematological study and determination of CD8, granzyme B enzyme and caspase-3 activities, MMP-(2&9) activities and protein fractionation in serum. Female mice are divided into: G1 is the control group. G2 is the Ehrlich treated group, injected with 2.5×105 viable tumor cells/mouse. G3 is vaccinated with xenogeneic placental extract (XPE) sterilized using 0.2 micron Millipore filters. G4 is vaccinated with XPE sterilized with gamma irradiation at dose level of 2 Gy. G3 and G4 are subdivided into a, b, c and d. Results demonstrated that subgroups 3a, 3c, 3d, 4a, 4b, 4c and 4d showed significant decrease in mean tumor size compared to G2. For lymphocyte count, subgroups 3d, 4b, 4c and 4d revealed a significant increase compared to G1. Subgroup 4d showed a significant increase in both CD8 and granzyme B activities compared to G1. The caspase-3 activity indicated significantly in subgroups 4c and 4d compared to G1. Serum MMP-(2&9) activities revealed that the 4d subgroup displays the less pronounced band compared to the other groups and to G2. Gel electrophoresis showed that Ehrlich protein and placental protein extract were represented by four bands detected at 7kDa, between 29kDa and 45kDa, at >67kDa and at >116kDa. It could be concluded that vaccination using xenogeneic placental extract targets the stimulation of the immune system. | ||||
| Keywords | ||||
| Immunotherapy; xenogeneic placental extract (XPE); cytotoxic T lymphocyte; Granzyme B; Caspase-3 | ||||
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