PROTECTIVE ROLE OF HONEY AFTER LEAD TOXICITY EFFECT ON ALBINO RATS LINGUAL PAPILLAE | ||||
| Alexandria Dental Journal | ||||
| Article 16, Volume 41, Issue 2, August 2016, Page 214-219 PDF (1.07 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjalexu.2016.59287 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| El shawakh Waleed O.* ; El-Sawa Afaf A.* ; Shafik Sahar K.* | ||||
| B.D.S. Faculty of Dentistry Triopli University | ||||
| Abstract | ||||
| INTRODUCTION: Studies concerning exposure of human to lead during daily activities are sometimes leading to many complications. Lead is a poisonous heavy metal, it’s toxicity can change the antioxidant balance in biological tissues. Honey is rich in phenolic acids and flavonoids, which exhibit a wide range of biological effects and act as natural antioxidants. OBJECTIVES: (1) To investigate the histological changes in lingual papillae following lead toxicity in experimental rats. (2) To assess the possible protective effects of honey using scanning electron microscopy (SEM). MATERIALS AND METHODS: Thirty two adult male albino rats were divided into four groups, eight rats/ group. Group I (control): 1ml distilled water. Group II (control + honey): 50 mg/kg honey + 1ml distilled water. Group III (lead): 16.5 mg lead acetate. Group IV (honey + lead): 16.5 mg lead acetate + 50 mg/kg honey. All doses were given by oral intubations daily /6 weeks. After 6 weeks, rats were sacrificed, and tongues were dissected out and processed for SEM. RESULTS: In Group II: Increased papillary density of the filiform papillae was noticed. In Group III the filiform papillae were distorted. Some appeared shorter while others had eroded tips and hyperkeratosis. Complete loss of papillae were seen in some samples. Disfigured fungiform papillae with swollen taste buds were seen. In Group IV, filiform and fungiform papillae appeared close to normal with minimal changes. CONCLUSIONS: The atrophy observed in Group III could be explained by lead-induced oxidative stress. Lead toxicity had a dual effect where increased free radical formation together with depletion of endogenous antioxidant enzymes resulted in inflammation and increased cell injury. Lead causes anemia and decreased salivary secretion, which may explain the epithelial erosion. Honey exerted its protective role through restoring enzymatic activity and through its antioxidant mechanism. | ||||
| Keywords | ||||
| Honey; filiform papillae; lead toxicity; fungiform papillae; SEM | ||||
| References | ||||
|
1. Moniuszko-Jakoniuk J, Brzoska M. Environment pollution and Health. Lek Wojsk. 1999; 75:419-426.
2. Aronson AL. Outbreaks of plumbism in animals associated with industrial lead operations. Clin Toxicol. 1972; 5:169-73.
3. Abdollahi M, Shohrati M, Nikfar SH, Jalali N. Monitoring of lead poisoning in bus drivers of tahran. Iran J Med Sci. 1995; 20:20-3.
4. Roché LM, Gerwel B, Ramaprasad R, Udasin IG. Medica management of lead-exposed workers: Results of physician interviews in New Jersey. J Occup Environ Med. 1995; 37:139-44.
5. Gurer H, Neal R, Yang P, Oztezcan S, Ercal N. Captopril as an antioxidant in lead-exposed Fischer 344 rats. Hum ExpToxicol. 1999; 18:27-32.
6. Watson GE, Davis BA, Raubertas RF, Pearson SK, Bowen WH. Influence of maternal lead ingestion on caries in rat pups. Nat Med. 1997; 3:1024-5.
7. Abdollahi M, Dehpour AR, Fooladgar M. Alteration of rat submandibulary gland secretion of protein, calcium and N-acetyl-beta- D-glucosaminidase activity by lead. Gen Pharmacol. 1997; 29:675-80.
8. Pincus D, Saccar CV. Lead poisoning. Am Fam Physician. 1979; 19:120-4.
9. Ciçek Y, Ertas U. The normal and pathological pigmentation of oral mucous membrane: A review. J Contemp Dent Pract. 2003; 4:76-8.
10. Tabrizizadeh M, Boozarjomehri F, AkhavanKarbasi MH, Maziar F. Evaluation of the relationship between blood lead level and prevalence of oral complications in Koushk lead mine workers, Yazd province. J Dent Med. 2006; 19:91-9.
11. El-Said KF, El-Ghamry AM, Mahdy NH, El-Bestawy NA. Chronic occupational exposure to lead and its impact on oral health. J Egypt Public Health Assoc. 2008; 83:451-66.
12. Gharzouli K, Gharzouli A, Amira S, Khennouf S. Prevention of ethanolinduced gastric lesions in rats by natural honey and glucose-fructosesucrose- maltose mixture. Pharmacol Res. 1999; 39:151-6.
13. Fathihah B, Mahmood AA, Sidik K, Salmah I. The antiulcer and cytoprotective effect of ageratum conyzoides – Honey combination in rats. JUMMEC. 2005; 8:28-32.
14. Echigo T, Takenaka T, Yatsunami K. Comparative studies on chemical composition of honey, royal jelly and pollen loads. Bull FacAgricTamagawaUniv. 1986; 26:1-12.
15.Bansal V, Medhi B, Pandhi P. Honey – a remedy rediscovered and its therapeutic utility. Kathmandu Univ Med J. 2005; 3:305-9.
16. Tandon SK, Singh S. Protection of lead-induced toxicity by hone in rats. Int J Pharmacognosy. 1994; 32:149-53.
17. Nanci A. Ten Cate’s Oral Histology Development, Structure and Function. 8th ed, St. Louis, MO: Mosby Publications; 2012. pp. 137-44.
18. Kaster AG, Cameron IL. Tongue selective habitation of bacteria on “intermediate soft” keratin surfaces of the rat. J Dent Res. 1978; 57:139.
19. Farbman AI. The dual pattern of keratinization in filiform papillae on rat tongue. J Anat. 1970; 106:233- 42.
20. Andrzejewska A, Szynaka B, Stokowska W. Ultra structural evaluation of the rat parotid gland after sixweek-intoxication with lead acetate. Mater Med Pol. 1994; 26:65-8.
21. Khadr ME, Mahdy KA, El-Shamy KA, Morsy FA, ElZayat SR, Abd-allah AA. Antioxidant activity and hepatoprotective potential of black seed, honey and silymarin on experimental liver injuries induced by CCL4 in rats. J ApplSci. 2007; 7:3909-17.
22. Nation JL. A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy. Stain Technol. 1983; 58:347-51.
23. OLPPP: Occupational Lead Poisoning Prevention Program. Medical Guidelines for the Lead-Exposed Worker. California: Occupational Health Branch, Department of Public Health; 2009. Available at: http://www. cdph.ca.gov/programs/olppp.
24. Osman HI, Abdel-Razek N, Koura SA. Histological changes of rat lingual papillae due to chromium toxicity and the protective role of vitamin E. Egypt Dent J. 2006; 52:193-200.
25.Jarvinen J, Kullaa-Mikkonen A, Kotilainen R. Some local and systemic factors related to tongue inflammation. Proc Finn Dent Soc. 1989; 85:199-209.
26. Suradkar SG, Vihol PD, Patel JH, Ghodasara DJ, Joshi DB, Prajapati KS. Patho-morphological changes in tissues of wistar rats by exposure of lead acetate. Vet World. 2010; 3:82-4.
27. Mogib El-Dahtory FA, Yahia S. Cytoprotective effect of honey against chromosomal breakage in fanconi anemia patients in vitro. Indian J Hum Genet. 2011; 17:77-81.
28. Needleman H. Lead poisoning. Annu Rev Med. 2004; 55:209-22.
29. Olaleye SB, Adaramoye OA, Erigbali PP Adeniyi OS. Lead exposure increases oxidative stress in the gastric mucosa of HCl/ethanol- exposed rats. World J Gastroenterol. 2007;13: 5121-6.
30. Sidhu P Nehru B. Lead intoxication: Histological and oxidative damage in rat cerebrum and cerebellum. J Trace Elem Exp Med. 2004; 17:45-53.
31. Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int. 2011;2011: 457327.
32. Abdollahi M, Fooladian F, Emami B, Zafari K, Bahreini-Moghadam A. Protection by sildenafil and theophylline of lead acetate-induced oxidative stress in rat submandibular gland and saliva. Hum ExpToxicol. 2003;22: 587-92.
33. Osaki T, Ueta E, Arisawa K, Kitamura Y, Matsugi N. The pathophysiology of glossal pain in patients with iron deficiency and anemia. Am J Med Sci. 1999;318: 324-9.
34. Patrick L. Lead toxicity, a review of the literature. Part 1: Exposure, evaluation, and treatment. Altern Med Rev. 2006; 11:2-22.
35. Anderson AC, Pueschel SM, Linakis JG. Pathophysiology of lead in lead poisoning. In: Pueschel S, Linakis J, Anderson A (eds). Lead Poisoning in Childhood. Baltimore: PH Brookes Publishing Co.; 1996. pp. 75-96.
36. Samaranayake L, Huber MA, Redding SW. Infectious disease. In: Greenberg M, Glick M, Ship JA, editors. Burkett's Oral Medicine, Diagnosis & Treatment, 11th ed. Hamilton: BC Decker Inc.; 2008. p. 490-1.
37. Fortoul TI, Velez-Cruz M, Antuna-Bizarro S, Montano LF, Rodriguez- Lara V Saldivar-Osorio L. Morphological changes in the tongue as a consequence of manganese inhalation in a murine experimental model: Light and scanning electron microscopic analysis. J Electron Microsc. 2010; 59:71-7.
38. Perez E, Rodriguez-Malaver AJ, Vit P. Antioxidant capacity of Venezuelan honey in wistar rat homogenates. J Med Food. 2006; 9:510-6.
39. Halawa HM, El-Nefiawy NE, Makhlouf NA, Mady AA. Evaluation of honey protective effect on lead induced oxidative stress in rats. JASMR. 2009; 4:197- 209.
40. Mohamed HA, El-Sayed IH, Moawad M. Protective effect of Nigella sativa seeds against dimethylaminoazobenzene (DAB) induced liver carcinogenesis. Nat Sci. 2010; 8:1-4 | ||||
|
Statistics Article View: 181 PDF Download: 555 |
||||
