Association of Plasmid-Mediated Quinolone Resistance with AmpC- Beta-Lactamase Producing E. coli strains from Different Sources | ||||
| Zagazig Veterinary Journal | ||||
| Article 3, Volume 42, Issue 1, March 2014, Page 19-30 PDF (4.72 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zvjz.2014.59465 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Norhan K Abd El-Aziz; Ahlam Abd El-Aziz | ||||
| Abstract | ||||
| Objectives: this study was devoted to detect the plasmid-mediated quinolone resistance (PMQR) mechanisms (qnr, qepA and aac(6')-Ib-cr ) and its association withAmpC- Beta-lactamase production in E. coli strains from different sources. Methods: Twenty-nine E. coli isolates from food-producing animals (chicken and sheep) and their by-products, collected from Sharkia province, Egypt were tested for their susceptibilities for different antimicrobial groups. Uniplex PCR was applied using specific primer sets for screening the presence of PMQR and AmpC β –lactamases genes in strains under study. Results: Out of 29 E. coli strains, only 9 isolates were positive for qnrA gene (31.03%) and associated with the amp < /em>C β -lactamase genes ( MOX, DHA, ACC, EBC) whereas only one qnrB and qnrS-like genes (3.45 %) were detected. A qnrB gene as well as qnrS was detected in (DHA , EBC) isolate. qepA and aac(6')-Ib-cr were detected in 41.38% and 3.45% of the E .coli isolates, respectively alone or in combination with qnr genes. The amp < /em>C β -lactamase genes were detected in 75.86 % of all strains and in 100% and 66.66 %of the PMQR determinant-positive and -negative strains, respectively, gave amplicons range from 302 bp to 520 bp, that easily distinguished by gel electrophoresis. Conclusions: A high prevalence of PMQR determinants among AmpC β-lactamase producing E. coli isolates from chicken mainly and their by-products was detected in Egypt. Their effect may slightly increase the MIC of quinolone and may relate to the development of full resistance to quinolone. | ||||
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