THE ROLE OF BCL-2 ONCOPROTEIN IN HEPATITIS C-RELATED HEPATOCELLULAR CARCINOMA | ||||
| Research Journal of Applied Biotechnology | ||||
| Article 11, Volume 2, Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology, November 2016, Page 101-110 PDF (863.58 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/rjab.2016.59641 | ||||
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| Authors | ||||
| Gehan, M. Ibrahim; Shaden Muawia; Mohamed El-Shahat; Tarek Kamel | ||||
| Molecular biology Dept., Genetic engineering and biotechnology research institute University of Sadat City, Egypt. | ||||
| Abstract | ||||
| Viral hepatitis has emerged as a major public health problem worldwide. There is increasing evidence that, several viral oncogenes such as core protein C virus (HCV) is playing a direct role in neoplastic transformation. The tumor development requires both increased proliferation and inhibition of apoptosis. There is increasing experimental evidence to suggest that bcl-2 plays a critical role in blocking apoptosis in cancer cells. In the present study we tried to explore the role of bcl-2 in development of viral hepatitis into hepatocellular carcinoma (HCC). Out of 105 participants; 50 patients were HCV positive infected patients, 30 HCC patients either infected with HCV (HCC/HCV) or not (HCC) and 25 healthy volunteers were also included. We determined the serum level of bcl-2, AFP and HCV-RNA viral load in patients and compared them with the other clinicopathological parameters. Increased serum bcl-2 level was showed in HCC/HCV infected patients more than HCV and controls (P=0.001) and more than HCC patients (P=0.018). A significant positive correlation between AFP and total load of viremia was elucidated (r=0.345, P=0.014) in the HCV infected patients who didn′t develop HCC. By analyzing the diagnostic performance of the studied parameters among the different groups. Bcl-2 was found to be a significant prognostic marker for the progression of HCC, as it represented two or more diagnostic values (Sensitivity 80 % , 70% and specificity 60%, 70% respectively).and (OR=6.0, 95% CI=1.74-20.59& OR=5.4 & 95% CI=1.75-16.88).These results make bcl-2 a candidate marker for HCC progression following HCV infection in Egyptian patients. | ||||
| Keywords | ||||
| Hepatocellular carcinoma (HCC) hepatitis C virus (HCV); Bcl-2 | ||||
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