Vitamin D Supports b-Cell Remodeling Capacity and Could Reduce High Fat Diet-Induced Insulinitis in Pups Born to Diabetic Mothers | ||||
| The Medical Journal of Cairo University | ||||
| Article 107, Volume 86, September, September 2018, Page 3003-3014 PDF (870.31 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2018.59867 | ||||
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| Authors | ||||
| ASMAA M. SHAMS EL-DEEN, M.D.; MANAL MOUSTAFA, M.D.; MARWA F. AMER, M.D.; GEORGE N. MORCOS, M.D.; HANAN ABDALLAH, M.D. | ||||
| The Departments of Physiology*, Medical Biochemistry & Molecular Biology** and Medical Histology***, Faculty of Medicine, Cairo University, Egypt | ||||
| Abstract | ||||
| Abstract Background: Disrupting fetal environment in particular the state of hyperglycemia during the critical period of pan-creatic development is one of potential factors that could alter b-cell function and morphology in adulthood. Vitamin D intake could protect b-cells from functional impairment and islet cells death. Aim of the Study: Present study aimed to study effect of maternal hyperglycemia on genes regulating insulin-secreting cells development and possible modulating actions of Vitamin D supplementation either during pregnancy or further contin-uation till weaning, also its possible protection from excess dietary fat intake. Methods: Twenty four adult female rats were mated and after confirmation of day 0 pregnancy, six of them were considered as control and the remaining eighteen were sub-jected to STZ-induced gestational diabetes then subdivided into pregnant-STZ, pregnant-STZ-supplied with Vitamin D during pregnancy and pregnant-STZ-supplied with Vitamin D during pregnancy and lactation till weaning. After weaning, pups born to those mothers were subjected to IPGTT then half of them were sacrificed and the rest were supplied with 45% HFD for two consecutive weeks. Results: The results observed up-regulation of Arx unlike Pax4 and Ngn3 which were down-regulated in pregnant-STZ group together with impairment of IPGTT and reduced area% of insulin immune-reactivity, these changes were augmented secondary to 45% intake of HFD. However, modulating impact of Vitamin D was more obvious in pregnant-STZ-supplied with Vitamin D during pregnancy and lactation than pregnant-STZ-supplied with Vitamin D only during pregnancy. Conclusion: Vitamin D supplementation till time of weaning could affect developmental programming of b-cells, enhance insulin secretion and protect against HFD-induced insulinitis. | ||||
| Keywords | ||||
| b-cell neogenesis – Vitamin D – Pax4 – Ngn3 – b-cell remodeling | ||||
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