Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test
IMA, S., Salama, E., S Abdel Rahman, N., M Ebied, E., A Ahmed, H., S El-Mahdy, S. (2014). Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test. EKB Journal Management System, 42(3), 217-224. doi: 10.21608/zvjz.2014.60195
Soliman IMA; Ekram Salama; Nehal S Abdel Rahman; Eman M Ebied; Hanan A Ahmed; Susan S El-Mahdy. "Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test". EKB Journal Management System, 42, 3, 2014, 217-224. doi: 10.21608/zvjz.2014.60195
IMA, S., Salama, E., S Abdel Rahman, N., M Ebied, E., A Ahmed, H., S El-Mahdy, S. (2014). 'Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test', EKB Journal Management System, 42(3), pp. 217-224. doi: 10.21608/zvjz.2014.60195
IMA, S., Salama, E., S Abdel Rahman, N., M Ebied, E., A Ahmed, H., S El-Mahdy, S. Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test. EKB Journal Management System, 2014; 42(3): 217-224. doi: 10.21608/zvjz.2014.60195

Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test

Zagazig Veterinary Journal
Article 21, Volume 42, Issue 3, September 2014, Page 217-224  XML PDF (3.12 MB)
Document Type: Original Article
DOI: 10.21608/zvjz.2014.60195
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Authors
Soliman IMA; Ekram Salama; Nehal S Abdel Rahman; Eman M Ebied; Hanan A Ahmed; Susan S El-Mahdy
Abstract
A trypsin-induced haemagglutination inhibition (THI) assay was standardized to achieve a quantification of haemagglutinating-inhibiting antibodies against infectious bronchitis virus (IBV) in the sera of chickens vaccinated with different types of commercial imported IBV vaccines. A total of forty four IBV vaccines representing as twenty two live vaccines containing either classical or variant strains and twenty two inactivated vaccines were examined among (THI) assay using trypsin treated classical IB antigen. In a trial for studying the correlation between the results of THI and the other serological tests (ELISA and serum neutralization test "SNT"). The former tests were performed on the same serum samples used in (THI) test. The results showed that the calculated mean HI titers for different examined serum samples ranged between 7.7 log2 to 7.9 log2, against live IB variants vaccines (CR88 and 4/91 respectively), 8.7 log2 to 9.2 log2 against classical type (Ma5, H120) vaccines, 9.1 log2 (H120+D274) and 6.8 log2 against inactivated vaccines. It was observed that quantification of antibodies in sera of chickens against infectious bronchitis virus by HI test had correlation with those obtained by either ELISA or SNT tests.
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